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Bloemberg D, Quadrilatero J. Effect of mitochondrial fission inhibition on C2C12 differentiation. Data Brief. 2016;7:634-40doi: 10.1016/j.dib.2016.02.070.
Bloemberg, D., & Quadrilatero, J. (2016). Effect of mitochondrial fission inhibition on C2C12 differentiation. Data in brief, 7634-40. https://doi.org/10.1016/j.dib.2016.02.070
Bloemberg, Darin, and Quadrilatero, Joe. "Effect of mitochondrial fission inhibition on C2C12 differentiation." Data in brief vol. 7 (2016): 634-40. doi: https://doi.org/10.1016/j.dib.2016.02.070
Bloemberg D, Quadrilatero J. Effect of mitochondrial fission inhibition on C2C12 differentiation. Data Brief. 2016 Mar 05;7:634-40. doi: 10.1016/j.dib.2016.02.070. eCollection 2016 Jun. PMID: 27054170; PMCID: PMC4802430.
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Data Brief. 2016 Mar 05;7:634-40. doi: 10.1016/j.dib.2016.02.070. eCollection 2016 Jun.

Effect of mitochondrial fission inhibition on C2C12 differentiation.

Data in brief

Darin Bloemberg, Joe Quadrilatero

Affiliations

  1. Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada.

PMID: 27054170 PMCID: PMC4802430 DOI: 10.1016/j.dib.2016.02.070

Abstract

The differentiation of skeletal muscle is commonly examined in cell culture using the C2C12 line of mouse skeletal myoblasts. This process shares many similarities with that which occurs during embryonic development, such as the transient activation of caspases. Here, we examined the effect of inhibiting mitochondrial fission, using mdivi-1, on the ability of C2C12 cells to terminally differentiate. This was performed using immunofluorescent identification of cell morphology and myosin expression, as well as immunoblotting for markers of muscle differentiation. Furthermore, the effect of mdivi-1 administration on activation of caspase-2 and -3 was assessed using spectrofluorometric measurement of specific enzyme activity.

Keywords: Apoptosis; C2C12; Caspases; Differentiation; Mitochondrial fission; Skeletal muscle

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