Display options
Cite
Yu ZG, Wang RG, Xiao C, et al. Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model. Evid Based Complement Alternat Med. 2016;2016:7074563doi: 10.1155/2016/7074563.
Yu, Z. G., Wang, R. G., Xiao, C., Zhao, J. Y., Shen, Q., Liu, S. Y., Xu, Q. W., Zhang, Q. X., & Wang, Y. T. (2016). Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model. Evidence-based complementary and alternative medicine : eCAM, 20167074563. https://doi.org/10.1155/2016/7074563
Yu, Zhan-Ge, et al. "Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model." Evidence-based complementary and alternative medicine : eCAM vol. 2016 (2016): 7074563. doi: https://doi.org/10.1155/2016/7074563
Yu ZG, Wang RG, Xiao C, Zhao JY, Shen Q, Liu SY, Xu QW, Zhang QX, Wang YT. Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model. Evid Based Complement Alternat Med. 2016;2016:7074563. doi: 10.1155/2016/7074563. Epub 2016 Apr 13. PMID: 27190536; PMCID: PMC4846758.
Copy Download .nbib Format: NLM
Share it on

Evid Based Complement Alternat Med. 2016;2016:7074563. doi: 10.1155/2016/7074563. Epub 2016 Apr 13.

Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model.

Evidence-based complementary and alternative medicine : eCAM

Zhan-Ge Yu, Rong-Guo Wang, Cheng Xiao, Jun-Yun Zhao, Qian Shen, Shou-Yao Liu, Qian-Wei Xu, Qing-Xi Zhang, Yun-Ting Wang

Affiliations

  1. Department of Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China.
  2. College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
  3. Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  4. School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China.
  5. Department of Tuina, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China.
  6. Department of Traditional Chinese Medicine Surgery, China-Japan Friendship Hospital, Beijing 100029, China.
  7. Department of Traditional Chinese Medicine, Northern Hospital, Beijing 100029, China.
  8. Department of Graduate School, Peking University of Health Science Center, Beijing 100029, China.
  9. Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, China.

PMID: 27190536 PMCID: PMC4846758 DOI: 10.1155/2016/7074563

Abstract

Objective. To explore the effects of electroacupuncture (EA) at ST36 (EA-ST36) and at Ashi acupoints (EA-Ashi) on skeletal muscle repair. Methods. Seventy-five rabbits were randomly divided into five groups: normal, contusion, EA-Ashi, EA-ST36, and EA at Ashi acupoints and ST36 (EA-AS). EA (0.4 mA, 2 Hz, 15 min) was applied after an acute gastrocnemius contusion. The morphology of myofibers and neuromuscular junctions (NMJs) and expressions of growth differentiation factor-8 (GDF-8), acetylcholinesterase (AChE), Neuregulin 1 (NGR1), and muscle-specific kinase (MuSK) were assessed 7, 14, and 28 days after contusion. Results. Compared with that in contusion group, there was an increase in the following respective parameters in treatment groups: the number and diameter of myofibers, the mean staining area, and continuities of NMJs. A comparison of EA-Ashi and EA-ST36 groups indicated that average myofiber diameter, mean staining area of NMJs, and expressions of AChE and NRG1 were higher in EA-Ashi group, whereas expression of GDF-8 decreased on day 7. However, increases in myofiber numbers, expressions of MuSK and AChE, as well as decreases in GDF-8 expression, and the discontinuities were observed in EA-ST36 group on the 28th day. Conclusion. Both EA-ST36 and EA-Ashi promoted myofiber regeneration and restoration of NMJs. EA-Ashi was more effective at earlier stages, whereas EA-ST36 played a more important role at later stages.

References

  1. Zhongguo Zhen Jiu. 2012 Jun;32(6):511-4 - PubMed
  2. Clin Orthop Relat Res. 1992 Oct;(283):296-301 - PubMed
  3. Neurochem Int. 2012 Dec;61(8):1397-403 - PubMed
  4. Sci Sin. 1979 Jun;22(6):680-92 - PubMed
  5. Neuron. 2004 Nov 18;44(4):578-80 - PubMed
  6. Biochim Biophys Acta. 2013 Oct;1834(10):2166-9 - PubMed
  7. J Cachexia Sarcopenia Muscle. 2012 Mar;3(1):13-23 - PubMed
  8. Evid Based Complement Alternat Med. 2013;2013:869398 - PubMed
  9. Br J Sports Med. 2013 Jun;47(9):556-60 - PubMed
  10. Am J Physiol Cell Physiol. 2002 Nov;283(5):C1557-65 - PubMed
  11. Handb Clin Neurol. 2008;91:103-48 - PubMed
  12. BMC Complement Altern Med. 2015 Mar 19;15:69 - PubMed
  13. Zhongguo Zhen Jiu. 2005 Nov;25(11):753-6 - PubMed
  14. J Vet Med Sci. 2010 Mar;72(3):279-84 - PubMed
  15. J Neurosci Methods. 2014 Apr 30;227:159-65 - PubMed
  16. ISRN Orthop. 2012 Apr 26;2012:689012 - PubMed
  17. J Agromedicine. 2014;19(3):268-80 - PubMed
  18. J Neuropathol Exp Neurol. 2004 Sep;63(9):919-31 - PubMed
  19. Chem Biol Interact. 2008 Sep 25;175(1-3):26-9 - PubMed
  20. Muscle Nerve. 2015 Mar;51(3):400-11 - PubMed
  21. J Biol Chem. 2007 Aug 31;282(35):25852-63 - PubMed
  22. J Surg Res. 2015 May 1;195(1):89-98 - PubMed
  23. J Acupunct Meridian Stud. 2011 Sep;4(3):153-8 - PubMed
  24. Chin J Integr Med. 2013 May;19(5):367-73 - PubMed
  25. J Acupunct Meridian Stud. 2014 Oct;7(5):238-42 - PubMed
  26. Acad Emerg Med. 2007 Jul;14(7):641-5 - PubMed
  27. Curr Top Dev Biol. 1970;5:235-80 - PubMed
  28. Mol Cell Neurosci. 2005 Feb;28(2):335-46 - PubMed
  29. Pain. 2008 Mar;135(1-2):11-9 - PubMed
  30. Burns. 2011 Feb;37(1):117-25 - PubMed
  31. Am J Phys Med Rehabil. 2009 Oct;88(10):815-22 - PubMed
  32. J Tradit Chin Med. 2015 Oct;35(5):499-506 - PubMed
  33. Anesth Analg. 2007 Feb;104(2):301-7 - PubMed
  34. J Tradit Chin Med. 2003 Jun;23(2):119-20 - PubMed
  35. J Physiol. 2013 Jan 15;591(2):559-70 - PubMed
  36. Neurosci Lett. 2013 Jun 17;545:29-34 - PubMed
  37. Neurochirurgie. 2009 Mar;55 Suppl 1:S49-62 - PubMed

Publication Types