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Lough F, Perry JD, Stanforth SP, et al. Determination of Carboxypeptidase Activity in Clinical Pathogens by Gas Chromatography-Mass Spectrometry. Anal Lett. 2016;49(8):1272-1277doi: 10.1080/00032719.2015.1092153.
Lough, F., Perry, J. D., Stanforth, S. P., & Dean, J. R. (2016). Determination of Carboxypeptidase Activity in Clinical Pathogens by Gas Chromatography-Mass Spectrometry. Analytical letters, 49(8), 1272-1277. https://doi.org/10.1080/00032719.2015.1092153
Lough, Fraser, et al. "Determination of Carboxypeptidase Activity in Clinical Pathogens by Gas Chromatography-Mass Spectrometry." Analytical letters vol. 49,8 (2016): 1272-1277. doi: https://doi.org/10.1080/00032719.2015.1092153
Lough F, Perry JD, Stanforth SP, Dean JR. Determination of Carboxypeptidase Activity in Clinical Pathogens by Gas Chromatography-Mass Spectrometry. Anal Lett. 2016 May 23;49(8):1272-1277. doi: 10.1080/00032719.2015.1092153. Epub 2016 Apr 21. PMID: 27226648; PMCID: PMC4867780.
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Anal Lett. 2016 May 23;49(8):1272-1277. doi: 10.1080/00032719.2015.1092153. Epub 2016 Apr 21.

Determination of Carboxypeptidase Activity in Clinical Pathogens by Gas Chromatography-Mass Spectrometry.

Analytical letters

Fraser Lough, John D Perry, Stephen P Stanforth, John R Dean

Affiliations

  1. Department of Applied Sciences, Northumbria University , Newcastle upon Tyne , UK.
  2. Department of Microbiology, Freeman Hospital , Newcastle upon Tyne , UK.

PMID: 27226648 PMCID: PMC4867780 DOI: 10.1080/00032719.2015.1092153

Abstract

A novel method for the determination of benzoic acid has been employed to identify carboxypeptidase activities in clinically relevant pathogens. Benzoic acid was determined after chemical derivatization by gas chromatography-mass spectrometry (GC-MS). N-Benzoyl amino acid substrates were evaluated for the detection of carboxypeptidase activities in a number of clinical pathogens. Upon enzymatic hydrolysis of these substrates, benzoic acid was produced which was detected by extraction from the liquid culture supernatant, derivatization as the trimethylsilyl ester, with subsequent analysis by GC-MS. Enzymatic hydrolysis of N-benzoyl glycine was observed for

Keywords: Carboxypeptidase activity; chemical derivatization; enzymatic hydrolysis; gas chromatography–mass spectrometry: N-benzoyl amino acid substrates

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