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Almeida F, Antoniêto AC, Pessoni AM, et al. Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells. Curr Genomics. 2016;17(2):112-8doi: 10.2174/1389202917666151116212705.
Almeida, F., Antoniêto, A. C., Pessoni, A. M., Monteiro, V. N., Alegre-Maller, A. C., Pigosso, L. L., Pereira, M., Soares, C. M., & Roque-Barreira, M. C. (2016). Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells. Current genomics, 17(2), 112-8. https://doi.org/10.2174/1389202917666151116212705
Almeida, Fausto, et al. "Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells." Current genomics vol. 17,2 (2016): 112-8. doi: https://doi.org/10.2174/1389202917666151116212705
Almeida F, Antoniêto AC, Pessoni AM, Monteiro VN, Alegre-Maller AC, Pigosso LL, Pereira M, Soares CM, Roque-Barreira MC. Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells. Curr Genomics. 2016 Apr;17(2):112-8. doi: 10.2174/1389202917666151116212705. PMID: 27226767; PMCID: PMC4864839.
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Curr Genomics. 2016 Apr;17(2):112-8. doi: 10.2174/1389202917666151116212705.

Influence of N-glycans on Expression of Cell Wall Remodeling Related Genes in Paracoccidioides brasiliensis Yeast Cells.

Current genomics

Fausto Almeida, Amanda Cristina Campos Antoniêto, André Moreira Pessoni, Valdirene Neves Monteiro, Ana Claudia Paiva Alegre-Maller, Laurine Lacerda Pigosso, Maristela Pereira, Célia Maria de Almeida Soares, Maria Cristina Roque-Barreira

Affiliations

  1. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP 14049-900, Brazil;
  2. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP 14040-900, Brazil;
  3. Universidade Estadural de Goiás, UnUCET, BR 153, Km98. Campus Henrique Santillo, Anapolis, GO 75000-000, Brazil;
  4. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.

PMID: 27226767 PMCID: PMC4864839 DOI: 10.2174/1389202917666151116212705

Abstract

Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America. It is caused by the temperature-dependent dimorphic fungus Paracoccidioides brasiliensis. The P. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and N-glycosylated proteins. These glycoproteins can interact with the host to affect infection rates, and are known to perform other functions. We inhibited N-linked glycosylation using tunicamycin (TM), and then evaluated the expression of P. brasiliensis genes related to cell wall remodeling. Our results suggest that cell wall synthesis related genes, such as β-1,3-glucanosyltransferase (PbGEL3), 1,3-β-D-glucan synthase (PbFKS1), and α-1,4-amylase (PbAMY), as well as cell wall degrading related genes, such as N-acetyl-β-D-glucosaminidase (PbNAG1), α-1,3-glucanase (PbAGN), and β-1,3-glucanase (PbBGN1 and PbBGN2), have their expression increased by the N-glycosylation inhibition, as detected by qRT-PCR. The observed increases in gene expression levels reveal possible compensatory mechanisms for diminished enzyme activity due to the lack of glycosylation caused by TM.

Keywords: Cell wall; Fungal cell.; N-glycan; Paracoccidioides brasiliensis

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