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Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, et al. Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur J Microbiol. 2016;9(3):e29057doi: 10.5812/jjm.29057.
Zarei Koosha, R., Mahmoodzadeh Hosseini, H., Mehdizadeh Aghdam, E., Ghorbani Tajandareh, S., & Imani Fooladi, A. A. (2016). Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur journal of microbiology, 9(3), e29057. https://doi.org/10.5812/jjm.29057
Zarei Koosha, Roohollah, et al. "Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens." Jundishapur journal of microbiology vol. 9,3 (2016): e29057. doi: https://doi.org/10.5812/jjm.29057
Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, Ghorbani Tajandareh S, Imani Fooladi AA. Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur J Microbiol. 2016 Mar 12;9(3):e29057. doi: 10.5812/jjm.29057. eCollection 2016 Mar. PMID: 27226873; PMCID: PMC4876617.
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Jundishapur J Microbiol. 2016 Mar 12;9(3):e29057. doi: 10.5812/jjm.29057. eCollection 2016 Mar.

Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens.

Jundishapur journal of microbiology

Roohollah Zarei Koosha, Hamideh Mahmoodzadeh Hosseini, Elnaz Mehdizadeh Aghdam, Shafie Ghorbani Tajandareh, Abbas Ali Imani Fooladi

Affiliations

  1. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran; Biology Department, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran.
  2. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
  3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IR Iran.

PMID: 27226873 PMCID: PMC4876617 DOI: 10.5812/jjm.29057

Abstract

BACKGROUND: Staphylococcus aureus is a harmful pathogen known to express numerous virulence factors and cause severe infections. High levels of methicillin-resistant Staphylococcus aureus (MRSA) strains are one of the important healthcare problems because of the inefficient treatment of these infections.

OBJECTIVES: The purpose of the current study is to evaluate the incidence of the toxic shock syndrome toxin (tsst-1) gene and its association with the prevalence of the mecA gene and drug resistance.

MATERIALS AND METHODS: The presence of the tsst-1 and mecA genes was investigated by polymerase chain reaction (PCR) among S. aureus isolated from 197 clinical samples. In addition, resistance tests to 12 antibiotics were carried out by the disc diffusion method.

RESULTS: Among the 197 isolates, 134 (68%) contained the tsst-1 genes and 172 (87.3%) contained the mecA genes. The prevalence of both genes was higher among male cases and samples purified from wounds and blood. We found no significant correlation between the presences of the two mentioned genes within isolates. The highest resistance we observed among our samples was to penicillin. None of isolates was resistant to vancomycin or linezolid. A significant correlation was observed between the presence of the mecA gene and resistance to oxacillin, gentamicin, kanamycin, erythromycin, tetracycline, cotrimoxazole, clindamycin, cephazolin and the multi-drug resistant property, which is resistance to more than three antibiotics (P < 0.05).

CONCLUSIONS: Our outcomes showed elevated incidences of tsst-1 positive and MRSA strains with higher rates of antibiotic resistance. The conflict between our findings and other records may be due to differences in geographic regions.

Keywords: MRSA; Staphylococcus aureus; mecA; tsst-1

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