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Touska F, Winter Z, Mueller A, et al. Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay. Temperature (Austin). 2016;3(1):77-91doi: 10.1080/23328940.2015.1135689.
Touska, F., Winter, Z., Mueller, A., Vlachova, V., Larsen, J., & Zimmermann, K. (2016). Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay. Temperature (Austin, Tex.), 3(1), 77-91. https://doi.org/10.1080/23328940.2015.1135689
Touska, Filip, et al. "Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay." Temperature (Austin, Tex.) vol. 3,1 (2016): 77-91. doi: https://doi.org/10.1080/23328940.2015.1135689
Touska F, Winter Z, Mueller A, Vlachova V, Larsen J, Zimmermann K. Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay. Temperature (Austin). 2016 Mar 02;3(1):77-91. doi: 10.1080/23328940.2015.1135689. eCollection 2016. PMID: 27227099; PMCID: PMC4861200.
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Temperature (Austin). 2016 Mar 02;3(1):77-91. doi: 10.1080/23328940.2015.1135689. eCollection 2016.

Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay.

Temperature (Austin, Tex.)

Filip Touska, Zoltán Winter, Alexander Mueller, Viktorie Vlachova, Jonas Larsen, Katharina Zimmermann

Affiliations

  1. Klinik für Anästhesiologie am Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany; Department of Cellular Neurophysiology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
  2. Klinik für Anästhesiologie am Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg , Erlangen, Germany.
  3. Department of Cellular Neurophysiology, Institute of Physiology of the Czech Academy of Sciences , Prague, Czech Republic.
  4. Independent scholar , Erlangen, Germany.

PMID: 27227099 PMCID: PMC4861200 DOI: 10.1080/23328940.2015.1135689

Abstract

Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes. To overcome current limitations we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts. Our custom-designed automated offline analysis by a blob detection algorithm is devoid of movement artifacts, removes light reflection artifacts and provides an internal quality control parameter which we validated. The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution. We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice.

Keywords: TRPA1; TRPM8; nociception; skew; thermal selection; thermosensation

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