Display options
Cite
Bardon J, Lukaszewicz AC, Faivre V, et al. Reactive oxygen species measure for rapid detection of infection in fluids. Ann Intensive Care. 2016;6(1):41doi: 10.1186/s13613-016-0142-8.
Bardon, J., Lukaszewicz, A. C., Faivre, V., Huot, B., & Payen, D. (2016). Reactive oxygen species measure for rapid detection of infection in fluids. Annals of intensive care, 6(1), 41. https://doi.org/10.1186/s13613-016-0142-8
Bardon, Jean, et al. "Reactive oxygen species measure for rapid detection of infection in fluids." Annals of intensive care vol. 6,1 (2016): 41. doi: https://doi.org/10.1186/s13613-016-0142-8
Bardon J, Lukaszewicz AC, Faivre V, Huot B, Payen D. Reactive oxygen species measure for rapid detection of infection in fluids. Ann Intensive Care. 2016 Dec;6(1):41. doi: 10.1186/s13613-016-0142-8. Epub 2016 Apr 29. PMID: 27130425; PMCID: PMC4851674.
Copy Download .nbib Format: NLM
Share it on

Ann Intensive Care. 2016 Dec;6(1):41. doi: 10.1186/s13613-016-0142-8. Epub 2016 Apr 29.

Reactive oxygen species measure for rapid detection of infection in fluids.

Annals of intensive care

Jean Bardon, Anne-Claire Lukaszewicz, Valérie Faivre, Benjamin Huot, Didier Payen

Affiliations

  1. Department of Anesthesiology and Critical Care and SAMU, Hôpital Lariboisière, AP-HP, Paris, France.
  2. Department of Anesthesiology and Critical Care and SAMU, Hôpital Lariboisière, AP-HP, Paris, France. [email protected].
  3. Université Paris Diderot, Sorbonne Paris Cité, Paris, France. [email protected].
  4. Inserm U1160, 75010, Paris, France. [email protected].
  5. Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
  6. Inserm U1160, 75010, Paris, France.

PMID: 27130425 PMCID: PMC4851674 DOI: 10.1186/s13613-016-0142-8

Abstract

BACKGROUND: Early detection of infection is critical to rapidly starting effective treatment. Diagnosis can be difficult, particularly in the intensive care unit (ICU) population. Because the presence of polymorphonuclear neutrophils in tissues is the hallmark of inflammatory processes, the objective of this proof of concept study was to determine whether the measurement of reactive oxygen species (ROS) could be an efficient diagnostic tool to rapidly diagnose infections in peritoneal, pleural and bronchoalveolar lavage (BAL) fluids in ICU patients.

METHODS: We prospectively included all patients hospitalized in the 21-bed surgical ICU of a teaching hospital from June 2010 to February 2014 who presented with systemic inflammatory response syndrome with suspicion of a peritoneal or pleural fluid or pulmonary infection needing a BAL. Instantaneous basal ROS production was measured in fluids and after phorbol 12-myristate 13-acetate (PMA) stimulation. We compared patients with infected fluids to those with non-infected fluids.

RESULTS: The overall ICU mortality rate was 34 %. A majority of patients were sampled following a delay of 5 days (2-12) after ICU admission, with most receiving antibiotics at the time of fluid sampling (71 %). Fluids were infected in 21/65 samples: 6/17 peritoneal fluids, 8/28 pleural fluids and 7/20 BALs. ROS production was significantly higher in the infected than in the non-infected group at baseline and after PMA stimulation in the peritoneal and pleural fluids but not in BAL.

CONCLUSION: Assessing instantaneous ROS production appears as a fast and reliable diagnostic method for detecting peritoneal and pleural fluid infection.

Keywords: Ascites; Bronchoalveolar lavage; Critical care; Diagnosis; Infection; Inflammation; Pleural effusion; Polymorphonuclear neutrophils; Reactive oxygen species

References

  1. Nature. 2011 Jan 13;469(7329):221-5 - PubMed
  2. PLoS One. 2014 Mar 21;9(3):e92564 - PubMed
  3. Lancet. 2006 Jul 8;368(9530):157-69 - PubMed
  4. Nat Immunol. 2002 Dec;3(12):1129-34 - PubMed
  5. Mech Ageing Dev. 2003 Apr;124(4):419-25 - PubMed
  6. Chest. 1997 Aug;112(2):445-57 - PubMed
  7. Immunity. 2014 Nov 20;41(5):694-707 - PubMed
  8. Nat Rev Immunol. 2014 Apr;14(4):232-46 - PubMed
  9. Annu Rev Immunol. 2005;23:197-223 - PubMed
  10. Nature. 2014 Nov 20;515(7527):431-5 - PubMed
  11. Hepatology. 2004 Mar;39(3):841-56 - PubMed
  12. Mediators Inflamm. 2011;2011:237638 - PubMed
  13. Crit Care. 2008;12(1):R25 - PubMed
  14. Antioxid Redox Signal. 2015 Feb 20;22(6):537-52 - PubMed
  15. Shock. 2003 Sep;20(3):208-12 - PubMed
  16. Clin Infect Dis. 1995 Dec;21 Suppl 3:S226-37 - PubMed
  17. N Engl J Med. 2005 Mar 3;352(9):865-74 - PubMed
  18. Neurochem Res. 2009 Jul;34(7):1255-60 - PubMed
  19. N Engl J Med. 2006 Dec 21;355(25):2619-30 - PubMed
  20. J Biol Chem. 1990 Nov 25;265(33):20241-6 - PubMed
  21. Pulm Med. 2012;2012:716235 - PubMed
  22. Free Radic Biol Med. 2008 Apr 1;44(7):1443-51 - PubMed
  23. World J Gastroenterol. 2011 Mar 7;17(9):1091-4 - PubMed
  24. Nat Rev Immunol. 2013 Mar;13(3):159-75 - PubMed
  25. PLoS One. 2015 May 26;10(5):e0128286 - PubMed
  26. Ann Intensive Care. 2012 Apr 10;2:10 - PubMed
  27. Ann Intern Med. 1972 Oct;77(4):507-13 - PubMed
  28. Respirology. 2004 Mar;9(1):4-11 - PubMed
  29. Nat Rev Drug Discov. 2009 Jul;8(7):579-91 - PubMed
  30. Adv Med Sci. 2014 Mar;59(1):52-6 - PubMed
  31. Crit Care Med. 2006 Jun;34(6):1589-96 - PubMed
  32. Intensive Care Med. 2002 Feb;28(2):108-21 - PubMed
  33. Gastroenterology. 2008 Sep;135(3):803-7 - PubMed
  34. Chest. 1997 Feb;111(2):411-8 - PubMed

Publication Types