Display options
Cite
Murad H, Hawat M, Ekhtiar A, et al. Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa. Cancer Cell Int. 2016;16:39doi: 10.1186/s12935-016-0315-4.
Murad, H., Hawat, M., Ekhtiar, A., AlJapawe, A., Abbas, A., Darwish, H., Sbenati, O., & Ghannam, A. (2016). Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa. Cancer cell international, 1639. https://doi.org/10.1186/s12935-016-0315-4
Murad, Hossam, et al. "Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa." Cancer cell international vol. 16 (2016): 39. doi: https://doi.org/10.1186/s12935-016-0315-4
Murad H, Hawat M, Ekhtiar A, AlJapawe A, Abbas A, Darwish H, Sbenati O, Ghannam A. Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa. Cancer Cell Int. 2016 May 26;16:39. doi: 10.1186/s12935-016-0315-4. eCollection 2016. PMID: 27231438; PMCID: PMC4881178.
Copy Download .nbib Format: NLM
Share it on

Cancer Cell Int. 2016 May 26;16:39. doi: 10.1186/s12935-016-0315-4. eCollection 2016.

Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa.

Cancer cell international

Hossam Murad, Mohammad Hawat, Adnan Ekhtiar, Abdulmunim AlJapawe, Assef Abbas, Hussein Darwish, Oula Sbenati, Ahmed Ghannam

Affiliations

  1. Division of Human Genetics, Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria.
  2. Division of Biochemistry & Toxicology, Department of Molecular Biology and Biotechnology, Damascus, Syria.
  3. Division of Mammalian Biology, Department of Molecular Biology and Biotechnology, Damascus, Syria.
  4. Laboratory of Marine biology, Faculty of Sciences, Tishreen University, Lattakia, Syria.
  5. Laboratory of plant functional genomics, Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria.

PMID: 27231438 PMCID: PMC4881178 DOI: 10.1186/s12935-016-0315-4

Abstract

BACKGROUND: Marine algae consumption is linked to law cancer incidences in countries that traditionally consume marine products. Hence, Phytochemicals are considered as potential chemo-preventive and chemotherapeutic agents against cancer. We investigated the effects of the algal sulfated polysaccharide extract (ASPE) from the red marine alga L. papillosa on MDA-MB-231 human breast cancer cell line.

METHODS: Flow cytometry analysis was performed to study the cell viability, cell cycle arrest and apoptosis. Changes in the expression of certain genes associated with cell cycle regulation was conducted by PCR real time analyses. Further investigations on apoptotic molecules was performed by ROS measurement and protein profiling.

RESULTS: ASPE at low doses (10 µg/ml), inhibited cell proliferation, and arrested proliferating MDA-MB-231 cells at G1-phase. However, higher doses (50 µg/ml), triggered apoptosis in those cells. The low dose of ASPE also caused up-regulation of Cip1/p21 and Kip1/p27 and down-regulation of cyclins D1, D2, and E1 transcripts and their related cyclin dependent kinases: Cdk2, Cdk4, and Cdk6. The higher doses of ASPE initiated a dose-dependent apoptotic death in MDA-MB-231 by induction of Bax transcripts, inhibition of Bcl-2 and cleavage of Caspase-3 protein. Over-generation of reactive oxygen species (ROS) were also observed in MDA-MB-231 treated cells.

CONCLUSIONS: These findings indicated that ASPE induces G1-phase arrest and apoptosis in MDA-MB-231 cells. ASPE may serve as a potential therapeutic agent for breast cancer.

Keywords: Apoptosis; G1-phase cell cycle arrest; MDA-MB-231; Red algae

References

  1. Nat Rev Mol Cell Biol. 2004 Nov;5(11):897-907 - PubMed
  2. Food Chem Toxicol. 2009 Jun;47(6):1378-84 - PubMed
  3. Cell. 1994 Jul 15;78(1):67-74 - PubMed
  4. Parasitol Res. 2011 Jul;109(1):47-52 - PubMed
  5. Eur J Pharmacol. 2012 Sep 5;690(1-3):22-30 - PubMed
  6. Anticancer Res. 2006 Nov-Dec;26(6B):4527-34 - PubMed
  7. J Biol Chem. 2005 Nov 18;280(46):38271-5 - PubMed
  8. Cancer Immunol Immunother. 2012 Jul;61(7):1113-24 - PubMed
  9. Nat Rev Cancer. 2002 Apr;2(4):277-88 - PubMed
  10. Nature. 1989 Dec 7;342(6250):705-8 - PubMed
  11. Free Radic Biol Med. 2012 Sep 15;53(6):1358-70 - PubMed
  12. Science. 2004 Jul 30;305(5684):626-9 - PubMed
  13. Nat Rev Cancer. 2003 Oct;3(10):768-80 - PubMed
  14. Food Chem Toxicol. 2006 Jul;44(7):1144-50 - PubMed
  15. Science. 2000 Mar 17;287(5460):1969-73 - PubMed
  16. Adv Food Nutr Res. 2011;64:225-33 - PubMed
  17. Cell. 1994 Nov 18;79(4):573-82 - PubMed
  18. Carbohydr Polym. 2013 Jan 30;92(1):577-82 - PubMed
  19. Breast Cancer Res Treat. 2002 Feb;71(3):269-80 - PubMed
  20. Int J Biol Macromol. 2013 Nov;62:70-5 - PubMed
  21. Fitoterapia. 2013 Jan;84:303-7 - PubMed
  22. Breast Cancer Res. 2004;6(6):262-8 - PubMed
  23. Int J Gynecol Cancer. 2006 Jan-Feb;16(1):165-70 - PubMed
  24. Saudi Pharm J. 2010 Jan;18(1):1-25 - PubMed
  25. Fitoterapia. 2011 Jul;82(5):742-9 - PubMed
  26. Nutr Cancer. 2003;45(2):236-46 - PubMed
  27. Int J Cancer. 2002 Jan 1;97(1):72-81 - PubMed
  28. J Cancer Prev. 2014 Dec;19(4):273-8 - PubMed
  29. Nature. 2000 Oct 12;407(6805):770-6 - PubMed
  30. J Mol Signal. 2008 Dec 01;3:18 - PubMed
  31. Cancer Cell Int. 2015 May 28;15:55 - PubMed
  32. Biol Res. 2010;43(1):31-7 - PubMed
  33. Mol Med Rep. 2015 Mar;11(3):2153-8 - PubMed
  34. Cancer Metastasis Rev. 1995 Mar;14(1):3-15 - PubMed
  35. Oncogene. 1995 Jul 20;11(2):211-9 - PubMed
  36. Curr Opin Cell Biol. 2000 Aug;12(4):414-9 - PubMed
  37. Int J Mol Sci. 2011;12(5):3352-65 - PubMed
  38. BMC Complement Altern Med. 2014 Oct 10;14:387 - PubMed
  39. J Mammary Gland Biol Neoplasia. 2007 Sep;12(2-3):153-62 - PubMed
  40. PLoS One. 2012;7(8):e43483 - PubMed
  41. Adv Food Nutr Res. 2012;66:75-145 - PubMed
  42. Tumour Biol. 2014 Jan;35(1):117-22 - PubMed
  43. Food Sci Technol Int. 2010 Oct;16(5):361-70 - PubMed
  44. Cancer Cell Int. 2015 Sep 29;15:90 - PubMed
  45. Toxicol Pathol. 2007 Jun;35(4):495-516 - PubMed
  46. PLoS One. 2010 Aug 12;5(8):e12124 - PubMed
  47. Br J Nutr. 2010 May;103(9):1345-53 - PubMed
  48. Int J Mol Med. 2009 Dec;24(6):801-6 - PubMed
  49. Fitoterapia. 2013 Jan;84:257-63 - PubMed
  50. Anticancer Res. 2006 Jul-Aug;26(4B):3049-55 - PubMed
  51. Genes Dev. 1999 Aug 1;13(15):1899-911 - PubMed
  52. Cancer Sci. 2010 Nov;101(11):2375-83 - PubMed
  53. Cancer Biol Ther. 2008 Jun;7(6):966-73 - PubMed
  54. PLoS One. 2010 Dec 14;5(12):e14320 - PubMed
  55. Cancer Cell Int. 2013 Jun 03;13(1):55 - PubMed
  56. Cancer Metastasis Rev. 1992 Sep;11(2):121-39 - PubMed
  57. Anticancer Res. 2008 Nov-Dec;28(6A):3677-89 - PubMed

Publication Types