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Miyazaki J, Iida T, Tanaka S, et al. Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy. Biomed Opt Express. 2016;7(5):1702-10doi: 10.1364/BOE.7.001702.
Miyazaki, J., Iida, T., Tanaka, S., Hayashi-Takagi, A., Kasai, H., Okabe, S., & Kobayashi, T. (2016). Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy. Biomedical optics express, 7(5), 1702-10. https://doi.org/10.1364/BOE.7.001702
Miyazaki, Jun, et al. "Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy." Biomedical optics express vol. 7,5 (2016): 1702-10. doi: https://doi.org/10.1364/BOE.7.001702
Miyazaki J, Iida T, Tanaka S, Hayashi-Takagi A, Kasai H, Okabe S, Kobayashi T. Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy. Biomed Opt Express. 2016 Apr 05;7(5):1702-10. doi: 10.1364/BOE.7.001702. eCollection 2016 May 01. PMID: 27231615; PMCID: PMC4871075.
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Biomed Opt Express. 2016 Apr 05;7(5):1702-10. doi: 10.1364/BOE.7.001702. eCollection 2016 May 01.

Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy.

Biomedical optics express

Jun Miyazaki, Tadatsune Iida, Shinji Tanaka, Akiko Hayashi-Takagi, Haruo Kasai, Shigeo Okabe, Takayoshi Kobayashi

Affiliations

  1. Advanced Ultrafast Laser Research Center, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan; JST, CREST, K' Gobancho, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
  2. Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyoku, Tokyo, 113-0033, Japan.
  3. Department of Structural Physiology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyoku, Tokyo, 113-0033, Japan.
  4. JST, CREST, K' Gobancho, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan; Department of Structural Physiology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyoku, Tokyo, 113-0033, Japan.
  5. Advanced Ultrafast Laser Research Center, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan; JST, CREST, K' Gobancho, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan; Department of Electrophysics, National Chiao-Tung University, Hsinchu 300, Taiwan; Institute of Laser Engineering, Osaka University, 2-6 Yamada-oka, Suita, Osaka 565-0971, Japan.

PMID: 27231615 PMCID: PMC4871075 DOI: 10.1364/BOE.7.001702

Abstract

A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 μs. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences.

Keywords: (170.6900) Three-dimensional microscopy; (350.5340) Photothermal effects

References

  1. Anal Bioanal Chem. 2007 Oct;389(4):1133-42 - PubMed
  2. Adv Nutr. 2013 Jul 01;4(4):474-6 - PubMed
  3. J Microsc. 2014 Nov;256(2):111-6 - PubMed
  4. Biophys J. 2012 Feb 8;102(3):672-81 - PubMed
  5. Opt Express. 2012 Sep 10;20(19):21385-99 - PubMed
  6. Mol Cell Neurosci. 2010 Jan;43(1):33-42 - PubMed
  7. Biomed Opt Express. 2012 Nov 1;3(11):2881-95 - PubMed
  8. Am J Clin Nutr. 2012 Nov;96(5):1161S-5S - PubMed
  9. Opt Express. 2007 Sep 17;15(19):12076-87 - PubMed
  10. Science. 2008 Dec 19;322(5909):1857-61 - PubMed
  11. J Biomed Opt. 2006 Jul-Aug;11(4):041120 - PubMed
  12. ACS Nano. 2011 Apr 26;5(4):2587-92 - PubMed
  13. Opt Express. 2007 Oct 17;15(21):14184-93 - PubMed
  14. Nat Methods. 2015 May;12(5):407-10 - PubMed
  15. Opt Express. 2009 Mar 2;17(5):3679-89 - PubMed
  16. Anal Bioanal Chem. 2004 Jan;378(1):60-2 - PubMed
  17. J Microsc. 2014 Jun;254(3):115-21 - PubMed
  18. Nano Lett. 2013 Apr 10;13(4):1489-94 - PubMed
  19. Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5970-5 - PubMed
  20. Anal Chem. 2014 Dec 16;86(24):12047-54 - PubMed
  21. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7081-6 - PubMed
  22. J Phys Chem Lett. 2012 May 17;3(10):1393-9 - PubMed
  23. Science. 2002 Aug 16;297(5584):1160-3 - PubMed
  24. Opt Lett. 2015 Feb 15;40(4):479-82 - PubMed
  25. Opt Express. 2015 Feb 9;23(3):3647-56 - PubMed
  26. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11350-5 - PubMed
  27. J Phys Chem Lett. 2012 May 17;3(10):1400-3 - PubMed
  28. J Biophotonics. 2010 Dec;3(12):791-806 - PubMed
  29. Biomed Opt Express. 2015 Aug 04;6(9):3217-24 - PubMed
  30. Anal Chem. 2002 Apr 1;74(7):1560-4 - PubMed

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