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Atul Jani R, Nag S, Setty SRG. Visualization of Intracellular Tyrosinase Activity . Bio Protoc. 2016;6(8)doi: 10.21769/bioprotoc.1794.
Atul Jani, R., Nag, S., & Setty, S. R. G. (2016). Visualization of Intracellular Tyrosinase Activity . Bio-protocol, 6(8), . https://doi.org/10.21769/bioprotoc.1794
Atul Jani, Riddhi, et al. "Visualization of Intracellular Tyrosinase Activity ." Bio-protocol vol. 6,8 (2016). doi: https://doi.org/10.21769/bioprotoc.1794
Atul Jani R, Nag S, Setty SRG. Visualization of Intracellular Tyrosinase Activity . Bio Protoc. 2016 Apr 20;6(8). doi: 10.21769/bioprotoc.1794. PMID: 27231711; PMCID: PMC4878704.
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Bio Protoc. 2016 Apr 20;6(8). doi: 10.21769/bioprotoc.1794.

Visualization of Intracellular Tyrosinase Activity .

Bio-protocol

Riddhi Atul Jani, Sudeshna Nag, Subba Rao Gangi Setty

Affiliations

  1. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.

PMID: 27231711 PMCID: PMC4878704 DOI: 10.21769/bioprotoc.1794

Abstract

Melanocytes produce the melanin pigments in melanosomes and these organelles protect the skin against harmful ultraviolet rays. Tyrosinase is the key cuproenzyme which initiates the pigment synthesis using its substrate amino acid tyrosine or L-DOPA (L-3, 4-dihydroxyphenylalanine). Moreover, the activity of tyrosinase directly correlates to the cellular pigmentation. Defects in tyrosinase transport to melanosomes or mutations in the enzyme or reduced intracellular copper levels results in loss of tyrosinase activity in melanosomes, commonly observed in albinism. Here, we described a method to detect the intracellular activity of tyrosinase in mouse melanocytes. This protocol will visualize the active tyrosinase present in the intracellular vesicles or organelles including melanosomes.

References

  1. Nature. 2008 Aug 28;454(7208):1142-6 - PubMed
  2. J Cell Sci. 2015 Sep 1;128(17):3263-76 - PubMed

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