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Marialouis XA, Santhanam A. Antibiotic Resistance, RAPD- PCR Typing of Multiple Drug Resistant Strains of Escherichia Coli From Urinary Tract Infection (UTI). J Clin Diagn Res. 2016;10(3):DC05-9doi: 10.7860/JCDR/2016/16470.7389.
Marialouis, X. A., & Santhanam, A. (2016). Antibiotic Resistance, RAPD- PCR Typing of Multiple Drug Resistant Strains of Escherichia Coli From Urinary Tract Infection (UTI). Journal of clinical and diagnostic research : JCDR, 10(3), DC05-9. https://doi.org/10.7860/JCDR/2016/16470.7389
Marialouis, Xavier Alexander, and Santhanam, Amutha. "Antibiotic Resistance, RAPD- PCR Typing of Multiple Drug Resistant Strains of Escherichia Coli From Urinary Tract Infection (UTI)." Journal of clinical and diagnostic research : JCDR vol. 10,3 (2016): DC05-9. doi: https://doi.org/10.7860/JCDR/2016/16470.7389
Marialouis XA, Santhanam A. Antibiotic Resistance, RAPD- PCR Typing of Multiple Drug Resistant Strains of Escherichia Coli From Urinary Tract Infection (UTI). J Clin Diagn Res. 2016 Mar;10(3):DC05-9. doi: 10.7860/JCDR/2016/16470.7389. Epub 2016 Mar 01. PMID: 27134870; PMCID: PMC4843256.
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J Clin Diagn Res. 2016 Mar;10(3):DC05-9. doi: 10.7860/JCDR/2016/16470.7389. Epub 2016 Mar 01.

Antibiotic Resistance, RAPD- PCR Typing of Multiple Drug Resistant Strains of Escherichia Coli From Urinary Tract Infection (UTI).

Journal of clinical and diagnostic research : JCDR

Xavier Alexander Marialouis, Amutha Santhanam

Affiliations

  1. Research Scholar, Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University , Madurai, Tamil Nadu, India .
  2. Professor, National Centre for Nanoscience and Nanotechnology, University of Madras , Guindy Campus, Chennai, Tamil Nadu, India .

PMID: 27134870 PMCID: PMC4843256 DOI: 10.7860/JCDR/2016/16470.7389

Abstract

INTRODUCTION: Global spreading of multidrug resistant strains of Escherichia coli is responsible for Urinary Tract Infection (UTI) which is a major health problem in of concern. Among the gram negative bacteria, the major contributors for UTI belongs to the family Enterobacteriaceae, which includes E. coli, Klebsiella, Citrobacter and Proteus. However, E. coli accounts for the major cause of Urinary tract infections (UTIs) and accounts for 75% to 90% of UTI isolates.

AIM: The main aim of this study is to analyse the phylogenetic grouping of clinical isolates of UTI E. coli.

MATERIALS AND METHODS: In this study nearly 58 E. coli strains were isolated and confirmed through microbiological, biochemical characterization. The urine samples were collected from outpatients having symptoms of UTI, irrespective of age and sex in Tamil Nadu, India. The isolates were subjected to analyse for ESBL and AmpC β-lactamase production. To understand its genetic correlation, molecular typing was carried out using RAPD-PCR method.

RESULTS: Here we noted phenotypically twenty seven isolates were positive for ESBL and seven for AmpC β-lactamase production. However, among the ESBL isolates higher sensitivity was noted for Nitrofurantoin and Cefoxitin. It is worth to note that the prevalence of UTIs was more common among female and elderly male. Phylogenetic grouping revealed the presence of 24 isolates belonged to B2 group followed by 19 isolates to group A, eight isolates to group B1 and Seven isolates to group D.

CONCLUSION: Phenotypically most of the strains were positive for ESBL and showed high sensitivity for Nitrofurantoin and cefoxitin.

Keywords: Antibiogram profiling; Molecular typing; Random amplified polymorphic DNA; β-lactamase

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