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Kazama D, Itakura M, Kurusu T, et al. Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis. Plants (Basel). 2013;2(4):769-85doi: 10.3390/plants2040769.
Kazama, D., Itakura, M., Kurusu, T., Mitsuda, N., Ohme-Takagi, M., & Tada, Y. (2013). Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis. Plants (Basel, Switzerland), 2(4), 769-85. https://doi.org/10.3390/plants2040769
Kazama, Daisuke, et al. "Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis." Plants (Basel, Switzerland) vol. 2,4 (2013): 769-85. doi: https://doi.org/10.3390/plants2040769
Kazama D, Itakura M, Kurusu T, Mitsuda N, Ohme-Takagi M, Tada Y. Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis. Plants (Basel). 2013 Dec 05;2(4):769-85. doi: 10.3390/plants2040769. PMID: 27137403; PMCID: PMC4844390.
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Plants (Basel). 2013 Dec 05;2(4):769-85. doi: 10.3390/plants2040769.

Identification of Chimeric Repressors that Confer Salt and Osmotic Stress Tolerance in Arabidopsis.

Plants (Basel, Switzerland)

Daisuke Kazama, Masateru Itakura, Takamitsu Kurusu, Nobutaka Mitsuda, Masaru Ohme-Takagi, Yuichi Tada

Affiliations

  1. Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. [email protected].
  2. School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. [email protected].
  3. School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. [email protected].
  4. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-0053, Japan. [email protected].
  5. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-0053, Japan. [email protected].
  6. Institute for Environmental Science and Technology, Saitama University, Saitama-shi, Saitama 338-8570, Japan. [email protected].
  7. School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. [email protected].

PMID: 27137403 PMCID: PMC4844390 DOI: 10.3390/plants2040769

Abstract

We produced transgenic Arabidopsis plants that express chimeric genes for transcription factors converted to dominant repressors, using Chimeric REpressor gene-Silencing Technology (CRES-T), and evaluated the salt tolerance of each line. The seeds of the CRES-T lines for ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23 exhibited higher germination rates than Wild type (WT) and developed rosette plants under up to 200 mM NaCl or 400 mM mannitol. WT plants did not grow under these conditions. In these CRES-T lines, the expression patterns of stress-related genes such as RD29A, RD22, DREB1A, and P5CS differed from those in WT plants, suggesting the involvement of the six transcription factors identified here in the stress response pathways regulated by the products of these stress-related genes. Our results demonstrate additional proof that CRES-T is a superior tool for revealing the function of transcription factors.

Keywords: CRES-T; osmotic stress; repressor; salt stress; stress tolerance; transcription factors

References

  1. Planta. 2006 Sep;224(4):878-88 - PubMed
  2. Nat Biotechnol. 2003 Jan;21(1):81-5 - PubMed
  3. Nat Biotechnol. 1999 Mar;17(3):287-91 - PubMed
  4. Biochem Biophys Res Commun. 2007 Dec 14;364(2):250-7 - PubMed
  5. Mol Biotechnol. 2013 May;54(1):102-23 - PubMed
  6. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11444-9 - PubMed
  7. Plant Physiol. 1996 Jan;110(1):249-257 - PubMed
  8. FEBS Lett. 2002 Mar 13;514(2-3):351-4 - PubMed
  9. Biochim Biophys Acta. 2009 Feb;1789(2):117-24 - PubMed
  10. Mol Plant Microbe Interact. 2007 Aug;20(8):900-11 - PubMed
  11. Plant Mol Biol. 2011 Jan;75(1-2):107-27 - PubMed
  12. Plant J. 2003 Jun;34(5):733-9 - PubMed
  13. Plant J. 2003 Jan;33(2):305-17 - PubMed
  14. Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3985-90 - PubMed
  15. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10308-13 - PubMed
  16. Biochem Biophys Res Commun. 2007 Nov 30;363(4):983-8 - PubMed
  17. Biochim Biophys Acta. 2007 Apr;1769(4):220-7 - PubMed
  18. Plant Cell. 1997 Oct;9(10):1859-68 - PubMed
  19. Plant Cell. 1994 Feb;6(2):251-64 - PubMed
  20. Science. 1999 Aug 20;285(5431):1256-8 - PubMed
  21. Plant Physiol. 2008 Feb;146(2):623-35 - PubMed
  22. Plant Mol Biol. 2008 Sep;68(1-2):119-29 - PubMed
  23. Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):12987-92 - PubMed
  24. Plant Biotechnol J. 2011 Sep;9(7):736-46 - PubMed
  25. Plant Physiol. 2006 Mar;140(3):1095-108 - PubMed
  26. Plant J. 2007 Apr;50(2):347-63 - PubMed
  27. Plant Cell. 1998 Aug;10(8):1391-406 - PubMed
  28. Plant Physiol. 2000 Dec;124(4):1854-65 - PubMed
  29. Plant J. 2004 Mar;37(5):720-9 - PubMed
  30. Plant Physiol. 2005 Sep;139(1):509-18 - PubMed
  31. Plant Mol Biol. 2008 May;67(1-2):169-81 - PubMed
  32. Plant J. 2008 Nov;56(4):613-26 - PubMed

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