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Gurunathan A, Senguttuvan J, Paulsamy S. Evaluation of Mosquito Repellent Activity of Isolated Oleic Acid, Eicosyl Ester from Thalictrum javanicum. Indian J Pharm Sci. 2016;78(1):103-10doi: 10.4103/0250-474x.180259.
Gurunathan, A., Senguttuvan, J., & Paulsamy, S. (2016). Evaluation of Mosquito Repellent Activity of Isolated Oleic Acid, Eicosyl Ester from Thalictrum javanicum. Indian journal of pharmaceutical sciences, 78(1), 103-10. https://doi.org/10.4103/0250-474x.180259
Gurunathan, Abinaya, et al. "Evaluation of Mosquito Repellent Activity of Isolated Oleic Acid, Eicosyl Ester from Thalictrum javanicum." Indian journal of pharmaceutical sciences vol. 78,1 (2016): 103-10. doi: https://doi.org/10.4103/0250-474x.180259
Gurunathan A, Senguttuvan J, Paulsamy S. Evaluation of Mosquito Repellent Activity of Isolated Oleic Acid, Eicosyl Ester from Thalictrum javanicum. Indian J Pharm Sci. 2016 Jan-Feb;78(1):103-10. doi: 10.4103/0250-474x.180259. PMID: 27168688; PMCID: PMC4852559.
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Indian J Pharm Sci. 2016 Jan-Feb;78(1):103-10. doi: 10.4103/0250-474x.180259.

Evaluation of Mosquito Repellent Activity of Isolated Oleic Acid, Eicosyl Ester from Thalictrum javanicum.

Indian journal of pharmaceutical sciences

Abinaya Gurunathan, Jamuna Senguttuvan, S Paulsamy

Affiliations

  1. Department of Botany, Kongunadu Arts and Science College, Coimbatore-641 029, India.

PMID: 27168688 PMCID: PMC4852559 DOI: 10.4103/0250-474x.180259

Abstract

To evaluate the traditional use, the mosquito repellent property of Thalictrum javanicum and to confirm the predicted larvicidal activity of the isolated compound, oleic acid, eicosyl ester from its aerial parts by PASS software, the present study was carried out using 4th instar stage larvae of the mosquitoes, Aedes aegypti (dengue vector) and Culex quinquefasciatus (filarial vector). Insecticidal susceptibility tests were conducted and the mortality rate was observed after 24 h exposure. The chitinase activity of isolated compound was assessed by using purified β-N-acetyl glucosaminidase (chitinase). Ecdysone 20-monooxygenase assay (radioimmuno assay) was made using the same larval stage of A. aegyptiand C. quinquefasciatus. The results were compared with the crude methanol extract of the whole plant. The isolated compound, oleic acid, eicosyl ester was found to be the most effective larvicide against A. aegypti (LC50/24 h -8.51 ppm) and C. quinquefasciatus (LC50/24 h - 12.5 ppm) than the crude methanol extract (LC50/24 h - 257.03 ppm and LC50/24 h - 281.83 ppm, respectively). The impact of oleic acid, eicosyl ester on reducing the activity of chitinase and ecdysone 20-monooxygenase was most prominent in both the target species, A. aegyptiand C. quinquefasciatus than the control. The results therefore suggest that the compound, oleic acid, eicosyl ester from Thalictrum javanicum may be considered as a potent source of mosquito larvicidal property.

Keywords: Thalictrum javanicum; aerial parts; eicosyl ester; larvicidal activity; oleic acid

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