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Tassinari E, Aznar S, Urcola I, et al. The incC Sequence Is Required for R27 Plasmid Stability. Front Microbiol. 2016;7:629doi: 10.3389/fmicb.2016.00629.
Tassinari, E., Aznar, S., Urcola, I., Prieto, A., Hüttener, M., & Juárez, A. (2016). The incC Sequence Is Required for R27 Plasmid Stability. Frontiers in microbiology, 7629. https://doi.org/10.3389/fmicb.2016.00629
Tassinari, Eleonora, et al. "The incC Sequence Is Required for R27 Plasmid Stability." Frontiers in microbiology vol. 7 (2016): 629. doi: https://doi.org/10.3389/fmicb.2016.00629
Tassinari E, Aznar S, Urcola I, Prieto A, Hüttener M, Juárez A. The incC Sequence Is Required for R27 Plasmid Stability. Front Microbiol. 2016 May 03;7:629. doi: 10.3389/fmicb.2016.00629. eCollection 2016. PMID: 27199955; PMCID: PMC4853401.
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Front Microbiol. 2016 May 03;7:629. doi: 10.3389/fmicb.2016.00629. eCollection 2016.

The incC Sequence Is Required for R27 Plasmid Stability.

Frontiers in microbiology

Eleonora Tassinari, Sonia Aznar, Imanol Urcola, Alejandro Prieto, Mário Hüttener, Antonio Juárez

Affiliations

  1. Institut de Bioenginyeria de Catalunya Barcelona, Spain.
  2. Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona Barcelona, Spain.
  3. Institut de Bioenginyeria de CatalunyaBarcelona, Spain; Departament de Microbiologia, Facultat de Biologia, Universitat de BarcelonaBarcelona, Spain.

PMID: 27199955 PMCID: PMC4853401 DOI: 10.3389/fmicb.2016.00629

Abstract

IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance.

Keywords: E protein; IncHI1 plasmids; antimicrobial resistance; incC; plasmid R27; plasmid stability

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