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Elistratova JG, Mustafina AR, Brylev KA, et al. Sensing activity of cholinesterases through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4.). Analyst. 2016;141(13):4204-10doi: 10.1039/c6an00581k.
Elistratova, J. G., Mustafina, A. R., Brylev, K. A., Petrov, K. A., Shestopalov, M. A., Mironov, Y. V., Babaev, V. M., Rizvanov, I. K., Masson, P., & Sinyashin, O. G. (2016). Sensing activity of cholinesterases through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4.). The Analyst, 141(13), 4204-10. https://doi.org/10.1039/c6an00581k
Elistratova, Julia G, et al. "Sensing activity of cholinesterases through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4.)." The Analyst vol. 141,13 (2016): 4204-10. doi: https://doi.org/10.1039/c6an00581k
Elistratova JG, Mustafina AR, Brylev KA, Petrov KA, Shestopalov MA, Mironov YV, Babaev VM, Rizvanov IK, Masson P, Sinyashin OG. Sensing activity of cholinesterases through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4.). Analyst. 2016 Jun 20;141(13):4204-10. doi: 10.1039/c6an00581k. PMID: 27169678.
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Analyst. 2016 Jun 20;141(13):4204-10. doi: 10.1039/c6an00581k.

Sensing activity of cholinesterases through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4.).

The Analyst

Julia G Elistratova, Asiya R Mustafina, Konstantin A Brylev, Konstantin A Petrov, Michael A Shestopalov, Yuri V Mironov, Vasily M Babaev, Ildar K Rizvanov, Patrick Masson, Oleg G Sinyashin

Affiliations

  1. A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, 8 Arbuzov str., 420088, Kazan, Russian Federation. [email protected].
  2. Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation.
  3. Kazan Federal University, 18 Kremlyovskaya str., 420008 Kazan, Russian Federation.

PMID: 27169678 DOI: 10.1039/c6an00581k

Abstract

The present work describes a new method to sense cholinesterase-catalyzed hydrolysis of acetylcholine (ACh) through a luminescence response of the hexarhenium cluster complex [{Re6S8}(OH)6](4-). A proton released from acetylcholinesterase (AChE)- or butyrylcholinesterase (BuChE)-catalyzed hydrolysis of ACh results in time-resolved sensitization of cluster-centered luminescence. The sensitization results from protonation of apical hydroxo-groups of the cluster complex. The protonation is affected by a counter ion effect. Thus, optimal conditions for adequate sensing of acetic acid produced by ACh hydrolysis are highlighted. Time-resolved luminescence and pH measurements under conditions of AChE-catalyzed hydrolysis of ACh show a good correlation between the cluster-centered luminescence and pH-induced inhibition of AChE. The inhibition is not significant within the first two minutes of ACh hydrolysis. Thus, the luminescence response measured within two minutes is dependent on both substrate and enzyme concentrations, which fits with AChE and BuChE kinetics. The usability of cluster-centered luminescence for monitoring the concentration-dependent inhibition of AChE with irreversible inhibitors is demonstrated, using a carbamylating agent, pyridostigmine bromide, as a model.

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