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Mendes NS, Silva PM, Silva-Rocha R, et al. Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa. FEBS Open Bio. 2016;6(4):358-68doi: 10.1002/2211-5463.12047.
Mendes, N. S., Silva, P. M., Silva-Rocha, R., Martinez-Rossi, N. M., & Rossi, A. (2016). Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa. FEBS open bio, 6(4), 358-68. https://doi.org/10.1002/2211-5463.12047
Mendes, Niege S, et al. "Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa." FEBS open bio vol. 6,4 (2016): 358-68. doi: https://doi.org/10.1002/2211-5463.12047
Mendes NS, Silva PM, Silva-Rocha R, Martinez-Rossi NM, Rossi A. Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa. FEBS Open Bio. 2016 Mar 14;6(4):358-68. doi: 10.1002/2211-5463.12047. eCollection 2016 Apr. PMID: 27239448; PMCID: PMC4821360.
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FEBS Open Bio. 2016 Mar 14;6(4):358-68. doi: 10.1002/2211-5463.12047. eCollection 2016 Apr.

Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa.

FEBS open bio

Niege S Mendes, Patricia M Silva, Rafael Silva-Rocha, Nilce M Martinez-Rossi, Antonio Rossi

Affiliations

  1. Department of Genetics Ribeirão Preto Medical School University of São Paulo Ribeirão Preto SP Brazil.
  2. Department of Molecular and Cellular Biology Ribeirão Preto Medical School University of São Paulo Ribeirão Preto SP Brazil.

PMID: 27239448 PMCID: PMC4821360 DOI: 10.1002/2211-5463.12047

Abstract

For this study, we sought to identify pre-mRNA processing events modulated by changes in extracellular pH, inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6-zinc finger regulator (fluffy), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling.

Keywords: Neurospora crassa; Pi regulation; alternative splicing; amphotericin B; asparagine synthetase; intron retention; ketoconazole

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