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Roedig P, Duman R, Sanchez-Weatherby J, et al. Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering. J Appl Crystallogr. 2016;49:968-975doi: 10.1107/S1600576716006348.
Roedig, P., Duman, R., Sanchez-Weatherby, J., Vartiainen, I., Burkhardt, A., Warmer, M., David, C., Wagner, A., & Meents, A. (2016). Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering. Journal of applied crystallography, 49968-975. https://doi.org/10.1107/S1600576716006348
Roedig, Philip, et al. "Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering." Journal of applied crystallography vol. 49 (2016): 968-975. doi: https://doi.org/10.1107/S1600576716006348
Roedig P, Duman R, Sanchez-Weatherby J, Vartiainen I, Burkhardt A, Warmer M, David C, Wagner A, Meents A. Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering. J Appl Crystallogr. 2016 May 23;49:968-975. doi: 10.1107/S1600576716006348. eCollection 2016 Jun 01. PMID: 27275143; PMCID: PMC4886986.
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J Appl Crystallogr. 2016 May 23;49:968-975. doi: 10.1107/S1600576716006348. eCollection 2016 Jun 01.

Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering.

Journal of applied crystallography

Philip Roedig, Ramona Duman, Juan Sanchez-Weatherby, Ismo Vartiainen, Anja Burkhardt, Martin Warmer, Christian David, Armin Wagner, Alke Meents

Affiliations

  1. Deutsches Elektronen-Synchrotron DESY, Photon Science, Notkestrasse 85, Hamburg 22607, Germany.
  2. Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, UK.
  3. Paul Scherrer Institut, Villigen PSI, 5232, Switzerland.

PMID: 27275143 PMCID: PMC4886986 DOI: 10.1107/S1600576716006348

Abstract

Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector technologies with high framing rates up to 1 kHz, fast sample exchange has become the bottleneck for such experiments. A micro-patterned chip has been developed from single-crystalline silicon, which acts as a sample holder for up to several thousand microcrystals at a very low background level. The crystals can be easily loaded onto the chip and excess mother liquor can be efficiently removed. Dehydration of the crystals is prevented by keeping them in a stream of humidified air during data collection. Further sealing of the sample holder, for example with Kapton, is not required. Room-temperature data collection from insulin crystals loaded onto the chip proves the applicability of the chip for macromolecular crystallography. Subsequent structure refinements reveal no radiation-damage-induced structural changes for insulin crystals up to a dose of 565.6 kGy, even though the total diffraction power of the crystals has on average decreased to 19.1% of its initial value for the same dose. A decay of the diffracting power by half is observed for a dose of

Keywords: X-ray radiation damage; crystallography on a chip; room-temperature crystallography; synchrotron serial crystallography

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