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Ibeyaima A, Rana J, Dwivedi A, et al. Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity. J Adv Pharm Technol Res. 2016;7(2):32-6doi: 10.4103/2231-4040.177201.
Ibeyaima, A., Rana, J., Dwivedi, A., Gupta, S., Sharma, S. K., Saini, N., & Sarethy, I. P. (2016). Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity. Journal of advanced pharmaceutical technology & research, 7(2), 32-6. https://doi.org/10.4103/2231-4040.177201
Ibeyaima, A, et al. "Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity." Journal of advanced pharmaceutical technology & research vol. 7,2 (2016): 32-6. doi: https://doi.org/10.4103/2231-4040.177201
Ibeyaima A, Rana J, Dwivedi A, Gupta S, Sharma SK, Saini N, Sarethy IP. Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity. J Adv Pharm Technol Res. 2016 Apr-Jun;7(2):32-6. doi: 10.4103/2231-4040.177201. PMID: 27144149; PMCID: PMC4850765.
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J Adv Pharm Technol Res. 2016 Apr-Jun;7(2):32-6. doi: 10.4103/2231-4040.177201.

Characterization of Yuhushiella sp. TD-032 from the Thar Desert and its antimicrobial activity.

Journal of advanced pharmaceutical technology & research

A Ibeyaima, Jyoti Rana, Anuj Dwivedi, Sanjay Gupta, Sanjeev K Sharma, Narendra Saini, Indira P Sarethy

Affiliations

  1. Department of Biotechnology, Jaypee Institute of Information Technology, Noida, India.
  2. Department of Microbiology, Pushpanjali Crosslay Hospital, Ghaziabad, Uttar Pradesh, India.

PMID: 27144149 PMCID: PMC4850765 DOI: 10.4103/2231-4040.177201

Abstract

During a screening program for antimicrobial compounds from underexplored habitats, a Gram-positive bacterium TD-032, was isolated from arid soil, Thar Desert (India), and analyzed for its morphological, physicochemical, and antimicrobial properties. The 16S ribosomal DNA (rDNA) sequence of the isolate was further studied for the novelty of γ-hyper variable region. TD-032 was grown in large-scale culture, and aqueous and organic solvent extracts analyzed for antimicrobial activity. Culture characteristics showed a lack of diffusible and melanoid pigments. The morphological features were pale yellow aerial mycelium colony color with brownish yellow substrate mycelium and leathery texture. The isolate could grow at 1% concentration of sodium chloride, temperature of 40°C, and a wide range of pH (7.0-12.0). An evaluation for extracellular enzymatic activities showed secretion of gelatinase(s), cellulase(s), and lipase(s). The γ-hyper variable region of 16S rDNA sequence of TD-032 showed 98.33% relatedness to Yuhushiella deserti, indicating a potential new species. Aqueous and ethyl acetate extracts showed antimicrobial activity against Gram-positive and Gram-negative bacteria inclusive clinical isolates. Inhibition of both test bacteria suggests that TD-032 produces a broad spectrum of antimicrobial substances.

Keywords: Actinomycetes; Thar Desert; antimicrobial activity; hyper-variable regions/16S ribosomal DNA

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