Front Microbiol. 2016 May 09;7:665. doi: 10.3389/fmicb.2016.00665. eCollection 2016.
Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench.
Frontiers in microbiology
Jonathan Tarn, Logan M Peoples, Kevin Hardy, James Cameron, Douglas H Bartlett
Affiliations
Affiliations
- Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.
- Global Ocean Dynamics, Global Ocean Design San Diego, CA, USA.
- Avatar Alliance Foundation Encino, CA, USA.
PMID: 27242695
PMCID: PMC4860528 DOI: 10.3389/fmicb.2016.00665
Abstract
Relatively few studies have described the microbial populations present in ultra-deep hadal environments, largely as a result of difficulties associated with sampling. Here we report Illumina-tag V6 16S rRNA sequence-based analyses of the free-living and particle-associated microbial communities recovered from locations within two of the deepest hadal sites on Earth, the Challenger Deep (10,918 meters below surface-mbs) and the Sirena Deep (10,667 mbs) within the Mariana Trench, as well as one control site (Ulithi Atoll, 761 mbs). Seawater samples were collected using an autonomous lander positioned ~1 m above the seafloor. The bacterial populations within the Mariana Trench bottom water samples were dissimilar to other deep-sea microbial communities, though with overlap with those of diffuse flow hydrothermal vents and deep-subsurface locations. Distinct particle-associated and free-living bacterial communities were found to exist. The hadal bacterial populations were also markedly different from one another, indicating the likelihood of different chemical conditions at the two sites. In contrast to the bacteria, the hadal archaeal communities were more similar to other less deep datasets and to each other due to an abundance of cosmopolitan deep-sea taxa. The hadal communities were enriched in 34 bacterial and 4 archaeal operational taxonomic units (OTUs) including members of the Gammaproteobacteria, Epsilonproteobacteria, Marinimicrobia, Cyanobacteria, Deltaproteobacteria, Gemmatimonadetes, Atribacteria, Spirochaetes, and Euryarchaeota. Sequences matching cultivated piezophiles were notably enriched in the Challenger Deep, especially within the particle-associated fraction, and were found in higher abundances than in other hadal studies, where they were either far less prevalent or missing. Our results indicate the importance of heterotrophy, sulfur-cycling, and methane and hydrogen utilization within the bottom waters of the deeper regions of the Mariana Trench, and highlight novel community features of these extreme habitats.
Keywords: dark biosphere; deep-sea microbiology; hadal; marine microbial diversity; piezophile
References
- Front Microbiol. 2013 Jul 24;4:182 - PubMed
- Nat Rev Microbiol. 2007 Oct;5(10):770-81 - PubMed
- Nat Commun. 2014 Nov 11;5:5385 - PubMed
- Appl Environ Microbiol. 2014 Jan;80(1):54-60 - PubMed
- Appl Environ Microbiol. 2006 Jul;72(7):5069-72 - PubMed
- Trends Ecol Evol. 2010 Mar;25(3):190-7 - PubMed
- Appl Environ Microbiol. 1999 Oct;65(10):4375-84 - PubMed
- Appl Environ Microbiol. 2000 Aug;66(8):3125-33 - PubMed
- Bioinformatics. 2014 Nov 1;30(21):3123-4 - PubMed
- Int J Syst Evol Microbiol. 2003 Nov;53(Pt 6):1801-5 - PubMed
- Int J Syst Evol Microbiol. 2009 Feb;59(Pt 2):350-6 - PubMed
- Science. 2007 Oct 5;318(5847):97-100 - PubMed
- ISME J. 2011 Mar;5(3):414-26 - PubMed
- PLoS One. 2014 May 16;9(5):e97876 - PubMed
- Curr Opin Chem Biol. 2003 Apr;7(2):160-5 - PubMed
- ISME J. 2009 Jul;3(7):860-9 - PubMed
- ISME J. 2015 Jun;9(6):1434-45 - PubMed
- Appl Environ Microbiol. 2002 Nov;68(11):5537-48 - PubMed
- Science. 1979 Aug 24;205(4408):808-10 - PubMed
- PLoS One. 2011;6(5):e20388 - PubMed
- Science. 2005 Mar 4;307(5714):1459-61 - PubMed
- ISME J. 2008 Jun;2(6):590-601 - PubMed
- Geobiology. 2012 Mar;10(2):178-92 - PubMed
- Sci Rep. 2012;2:270 - PubMed
- Science. 2006 Jan 27;311(5760):496-503 - PubMed
- Appl Environ Microbiol. 2009 Aug;75(16):5345-55 - PubMed
- J Biotechnol. 2006 Oct 20;126(1):11-25 - PubMed
- Appl Environ Microbiol. 2015 Dec;81(24):8265-76 - PubMed
- ISME J. 2013 Jan;7(1):161-72 - PubMed
- Extremophiles. 2006 Jun;10(3):181-9 - PubMed
- Genome Biol. 2007;8(7):R143 - PubMed
- Bioinformatics. 2011 Aug 15;27(16):2194-200 - PubMed
- Nat Rev Microbiol. 2014 Oct;12(10):686-98 - PubMed
- Nature. 2006 Feb 16;439(7078):847-50 - PubMed
- Environ Microbiol. 2008 Aug;10 (8):2138-49 - PubMed
- Science. 2008 Oct 10;322(5899):275-8 - PubMed
- Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2831-5 - PubMed
- ISME J. 2010 Feb;4(2):159-70 - PubMed
- ISME J. 2013 Feb;7(2):256-68 - PubMed
- Appl Environ Microbiol. 1999 Dec;65(12):5554-63 - PubMed
- Appl Environ Microbiol. 1997 Jan;63(1):50-6 - PubMed
- Appl Environ Microbiol. 2005 Jan;71(1):467-79 - PubMed
- ISME J. 2008 Aug;2(8):865-86 - PubMed
- Environ Microbiol. 2015 Oct;17(10):3481-99 - PubMed
- Appl Environ Microbiol. 2002 Feb;68(2):661-7 - PubMed
- PLoS One. 2014 May 05;9(5):e96449 - PubMed
- Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17633-8 - PubMed
- Front Microbiol. 2013 Apr 17;4:85 - PubMed
- Microb Ecol. 2011 Oct;62(3):549-63 - PubMed
- Appl Environ Microbiol. 2005 Jul;71(7):3495-503 - PubMed
- Environ Microbiol. 2001 Jan;3(1):72-8 - PubMed
- Front Microbiol. 2013 May 30;4:120 - PubMed
- Appl Environ Microbiol. 2004 Feb;70(2):781-9 - PubMed
- ISME J. 2013 Oct;7(10):1962-73 - PubMed
- Nat Rev Microbiol. 2006 Jun;4(6):458-68 - PubMed
- Environ Microbiol Rep. 2011 Aug;3(4):449-58 - PubMed
- Nucleic Acids Res. 2007;35(21):7188-96 - PubMed
- ISME J. 2009 Jul;3(7):780-91 - PubMed
- Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):E1230-6 - PubMed
- FEMS Microbiol Ecol. 2000 Aug 1;33(2):89-99 - PubMed
- Mar Biotechnol (NY). 2001 Sep;3(5):454-62 - PubMed
- Nat Commun. 2014;5:3192 - PubMed
- Mar Biotechnol (NY). 2005 Jul-Aug;7(4):253-71 - PubMed
- Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):12115-20 - PubMed
- Extremophiles. 2008 Jan;12(1):15-25 - PubMed
- Nat Commun. 2015 Jul 09;6:7608 - PubMed
- Environ Microbiol. 2008 Sep;10(9):2313-30 - PubMed
- ISME J. 2014 Oct;8(10):2029-44 - PubMed
- BMC Bioinformatics. 2014 Feb 05;15:41 - PubMed
- Environ Microbiol Rep. 2011 Dec;3(6):689-97 - PubMed
- PLoS One. 2007 Sep 19;2(9):e914 - PubMed
- Extremophiles. 1997 Aug;1(3):117-23 - PubMed
- Front Microbiol. 2013 Jun 17;4:153 - PubMed
- Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22427-32 - PubMed
- FEMS Microbiol Lett. 1999 Jan 1;170(1):271-9 - PubMed
- FEMS Microbiol Ecol. 2013 Sep;85(3):537-52 - PubMed
- ISME J. 2015 Aug;9(8):1747-63 - PubMed
- Appl Environ Microbiol. 2009 Dec;75(23):7537-41 - PubMed
- Appl Environ Microbiol. 2010 Sep;76(17):5902-10 - PubMed
- Front Microbiol. 2013 May 21;4:124 - PubMed
- FEMS Microbiol Ecol. 2015 Jan;91(1):1-11 - PubMed
- Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2247-53 - PubMed
- Appl Environ Microbiol. 1988 Jun;54(6):1426-9 - PubMed
- Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3195-201 - PubMed
- ISME J. 2015 Jul;9(7):1619-34 - PubMed
- Appl Environ Microbiol. 2009 Jun;75(11):3407-18 - PubMed
- Appl Environ Microbiol. 2000 May;66(5):1777-87 - PubMed
- ISME J. 2008 Dec;2(12):1194-212 - PubMed
- J Bacteriol. 1999 Aug;181(15):4517-25 - PubMed
Publication Types