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Iguchi A, Iyoda S, Seto K, et al. Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli. Front Microbiol. 2016;7:765doi: 10.3389/fmicb.2016.00765.
Iguchi, A., Iyoda, S., Seto, K., Nishii, H., Ohnishi, M., Mekata, H., Ogura, Y., & Hayashi, T. (2016). Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli. Frontiers in microbiology, 7765. https://doi.org/10.3389/fmicb.2016.00765
Iguchi, Atsushi, et al. "Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli." Frontiers in microbiology vol. 7 (2016): 765. doi: https://doi.org/10.3389/fmicb.2016.00765
Iguchi A, Iyoda S, Seto K, Nishii H, Ohnishi M, Mekata H, Ogura Y, Hayashi T. Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli. Front Microbiol. 2016 May 20;7:765. doi: 10.3389/fmicb.2016.00765. eCollection 2016. PMID: 27242776; PMCID: PMC4873512.
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Front Microbiol. 2016 May 20;7:765. doi: 10.3389/fmicb.2016.00765. eCollection 2016.

Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli.

Frontiers in microbiology

Atsushi Iguchi, Sunao Iyoda, Kazuko Seto, Hironobu Nishii, Makoto Ohnishi, Hirohisa Mekata, Yoshitoshi Ogura, Tetsuya Hayashi

Affiliations

  1. Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki Miyazaki, Japan.
  2. Department of Bacteriology I, National Institute of Infectious Diseases Tokyo, Japan.
  3. Division of Bacteriology, Osaka Prefectural Institute of Public Health Osaka, Japan.
  4. Organization for Promotion of Tenure Track, University of MiyazakiMiyazaki, Japan; Center for Animal Disease Control, University of MiyazakiMiyazaki, Japan.
  5. Department of Bacteriology, Faculty of Medical Sciences, Kyushu University Fukuoka, Japan.

PMID: 27242776 PMCID: PMC4873512 DOI: 10.3389/fmicb.2016.00765

Abstract

Serotyping is one of the typing techniques used to classify strains within the same species. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes. In a previous study, based on the O-antigen biosynthesis gene cluster (O-AGC) sequences of 184 known Escherichia coli O serogroups (from O1 to O187), we developed a comprehensive and practical molecular O serogrouping (O genotyping) platform using a polymerase chain reaction (PCR) method, named E. coli O-genotyping PCR. Although, the validation assay using the PCR system showed that most of the tested strains were successfully classified into one of the O genotypes, it was impossible to classify 6.1% (35/575) of the strains, suggesting the presence of novel O genotypes. In this study, we conducted sequence analysis of O-AGCs from O-genotype untypeable Shiga toxin-producing E. coli (STEC) strains and identified six novel O genotypes; OgN1, OgN8, OgN9, OgN10, OgN12 and OgN31, with unique wzx and/or wzy O-antigen processing gene sequences. Additionally, to identify these novel O-genotypes, we designed specific PCR primers. A screen of O genotypes using O-genotype untypeable strains showed 13 STEC strains were classified into five novel O genotypes. The O genotyping at the molecular level of the O-AGC would aid in the characterization of E. coli isolates and will assist future studies in STEC epidemiology and phylogeny.

Keywords: E. coli; O serogroup; PCR; STEC; genotyping techniques

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