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Ali Nasir A, Younus M, Rashid A, et al. Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes. Iran J Vet Res. 2015;16(1):94-9
Ali Nasir, A., Younus, M., Rashid, A., Abdul Khaliq, S., Khan, E., Shah, S. H., Aslam, A., Ghumman, M. A., & Joiya, M. H. (2015). Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes. Iranian journal of veterinary research, 16(1), 94-9.
Ali Nasir, A, et al. "Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes." Iranian journal of veterinary research vol. 16,1 (2015): 94-9.
Ali Nasir A, Younus M, Rashid A, Abdul Khaliq S, Khan E, Shah SH, Aslam A, Ghumman MA, Joiya MH. Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes. Iran J Vet Res. 2015;16(1):94-9. PMID: 27175159; PMCID: PMC4789248.
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Iran J Vet Res. 2015;16(1):94-9.

Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes.

Iranian journal of veterinary research

A Ali Nasir, M Younus, A Rashid, S Abdul Khaliq, E Khan, S H Shah, A Aslam, M A Ghumman, M H Joiya

Affiliations

  1. Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan;
  2. Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan;
  3. Department of Media Sterilization, University of Veterinary and Animal Sciences, Lahore, Pakistan.

PMID: 27175159 PMCID: PMC4789248

Abstract

The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histological examination revealed edema of lungs, kidney, and lymph nodes and to some extent in brain along with hemorrhages in lungs and intestines. Clostridium perfringens type D carrying alpha and epsilon toxin genes were amplified with amplicon size about 247 bp and 665 bp, respectively. Human erythrocytes showed the highest hemolysis, 68%, followed by mice, 57%, against culture supernatants. The percentage of hemolysis was significantly higher at 37°C as compared to 25°C except for rabbit and dog.

Keywords: Alpha and Epsilon genes; Clostridium perfringens type D; Enterotoxaemia ELISA; Hemolysis

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