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Kubo K, Paape T, Hatakeyama M, et al. Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia. Nat Plants. 2015;1:14005doi: 10.1038/nplants.2014.5.
Kubo, K., Paape, T., Hatakeyama, M., Entani, T., Takara, A., Kajihara, K., Tsukahara, M., Shimizu-Inatsugi, R., Shimizu, K. K., & Takayama, S. (2015). Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia. Nature plants, 114005. https://doi.org/10.1038/nplants.2014.5
Kubo, Ken-Ichi, et al. "Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia." Nature plants vol. 1 (2015): 14005. doi: https://doi.org/10.1038/nplants.2014.5
Kubo K, Paape T, Hatakeyama M, Entani T, Takara A, Kajihara K, Tsukahara M, Shimizu-Inatsugi R, Shimizu KK, Takayama S. Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia. Nat Plants. 2015 Jan 08;1:14005. doi: 10.1038/nplants.2014.5. PMID: 27246052.
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Nat Plants. 2015 Jan 08;1:14005. doi: 10.1038/nplants.2014.5.

Gene duplication and genetic exchange drive the evolution of S-RNase-based self-incompatibility in Petunia.

Nature plants

Ken-Ichi Kubo, Timothy Paape, Masaomi Hatakeyama, Tetsuyuki Entani, Akie Takara, Kie Kajihara, Mai Tsukahara, Rie Shimizu-Inatsugi, Kentaro K Shimizu, Seiji Takayama

Affiliations

  1. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
  2. Institute of Evolutionary Biology and Environmental Studies, University of Zurich, CH-8057 Zurich, Switzerland.
  3. Functional Genomics Center Zurich, CH-8057 Zurich, Switzerland.

PMID: 27246052 DOI: 10.1038/nplants.2014.5

Abstract

Self-incompatibility (SI) systems in flowering plants distinguish self- and non-self pollen to prevent inbreeding. While other SI systems rely on the self-recognition between specific male- and female-determinants, the Solanaceae family has a non-self recognition system resulting in the detoxification of female-determinants of S-ribonucleases (S-RNases), expressed in pistils, by multiple male-determinants of S-locus F-box proteins (SLFs), expressed in pollen. It is not known how many SLF components of this non-self recognition system there are in Solanaceae species, or how they evolved. We identified 16-20 SLFs in each S-haplotype in SI Petunia, from a total of 168 SLF sequences using large-scale next-generation sequencing and genomic polymerase chain reaction (PCR) techniques. We predicted the target S-RNases of SLFs by assuming that a particular S-allele must not have a conserved SLF that recognizes its own S-RNase, and validated these predictions by transformation experiments. A simple mathematical model confirmed that 16-20 SLF sequences would be adequate to recognize the vast majority of target S-RNases. We found evidence of gene conversion events, which we suggest are essential to the constitution of a non-self recognition system and also contribute to self-compatible mutations.

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