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Shi L, Ravikumar V, Derouiche A, et al. Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival. Front Microbiol. 2016;7:533doi: 10.3389/fmicb.2016.00533.
Shi, L., Ravikumar, V., Derouiche, A., Macek, B., & Mijakovic, I. (2016). Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival. Frontiers in microbiology, 7533. https://doi.org/10.3389/fmicb.2016.00533
Shi, Lei, et al. "Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival." Frontiers in microbiology vol. 7 (2016): 533. doi: https://doi.org/10.3389/fmicb.2016.00533
Shi L, Ravikumar V, Derouiche A, Macek B, Mijakovic I. Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival. Front Microbiol. 2016 Apr 19;7:533. doi: 10.3389/fmicb.2016.00533. eCollection 2016. PMID: 27148221; PMCID: PMC4835898.
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Front Microbiol. 2016 Apr 19;7:533. doi: 10.3389/fmicb.2016.00533. eCollection 2016.

Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival.

Frontiers in microbiology

Lei Shi, Vaishnavi Ravikumar, Abderahmane Derouiche, Boris Macek, Ivan Mijakovic

Affiliations

  1. Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology Gothenburg, Sweden.
  2. Proteome Center Tübingen, Interfaculty Institute for Cell Biology, University of Tübingen Tübingen, Germany.

PMID: 27148221 PMCID: PMC4835898 DOI: 10.3389/fmicb.2016.00533

Abstract

In order to screen for cellular substrates of the Bacillus subtilis BY-kinase PtkA, and its cognate phosphotyrosine-protein phosphatase PtpZ, we performed a triple Stable Isotope Labeling by Amino acids in Cell culture-based quantitative phosphoproteome analysis. Detected tyrosine phosphorylation sites for which the phosphorylation level decreased in the ΔptkA strain and increased in the ΔptpZ strain, compared to the wild type (WT), were considered as potential substrates of PtkA/PtpZ. One of those sites was the residue tyrosine 601 of the molecular chaperone DnaK. We confirmed that DnaK is a substrate of PtkA and PtpZ by in vitro phosphorylation and dephosphorylation assays. In vitro, DnaK Y601F mutant exhibited impaired interaction with its co-chaperones DnaJ and GrpE, along with diminished capacity to hydrolyze ATP and assist the re-folding of denatured proteins. In vivo, loss of DnaK phosphorylation in the mutant strain dnaK Y601F, or in the strain overexpressing the phosphatase PtpZ, led to diminished survival upon heat shock, consistent with the in vitro results. The decreased survival of the mutant dnaK Y601F at an elevated temperature could be rescued by complementing with the WT dnaK allele expressed ectopically. We concluded that the residue tyrosine 601 of DnaK can be phosphorylated and dephosphorylated by PtkA and PtpZ, respectively. Furthermore, Y601 is important for DnaK chaperone activity and heat shock survival of B. subtilis.

Keywords: SILAC; bacterial protein-tyrosine kinases; molecular chaperones; protein folding; protein phosphorylation; quantitative phosphoproteomics

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