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He JW, Hassan YI, Perilla N, et al. Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions. Front Microbiol. 2016;7:572doi: 10.3389/fmicb.2016.00572.
He, J. W., Hassan, Y. I., Perilla, N., Li, X. Z., Boland, G. J., & Zhou, T. (2016). Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions. Frontiers in microbiology, 7572. https://doi.org/10.3389/fmicb.2016.00572
He, Jian Wei, et al. "Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions." Frontiers in microbiology vol. 7 (2016): 572. doi: https://doi.org/10.3389/fmicb.2016.00572
He JW, Hassan YI, Perilla N, Li XZ, Boland GJ, Zhou T. Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions. Front Microbiol. 2016 Apr 21;7:572. doi: 10.3389/fmicb.2016.00572. eCollection 2016. PMID: 27148248; PMCID: PMC4838601.
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Front Microbiol. 2016 Apr 21;7:572. doi: 10.3389/fmicb.2016.00572. eCollection 2016.

Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions.

Frontiers in microbiology

Jian Wei He, Yousef I Hassan, Norma Perilla, Xiu-Zhen Li, Greg J Boland, Ting Zhou

Affiliations

  1. Guelph Research and Development Centre, Agriculture and Agri-Food Canada, GuelphON, Canada; School of Environmental Sciences, University of Guelph, GuelphON, Canada.
  2. Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph ON, Canada.
  3. Guelph Research and Development Centre, Agriculture and Agri-Food Canada, GuelphON, Canada; Micotox Ltd.Bogota, Colombia.
  4. School of Environmental Sciences, University of Guelph, Guelph ON, Canada.

PMID: 27148248 PMCID: PMC4838601 DOI: 10.3389/fmicb.2016.00572

Abstract

Deoxynivalenol (DON) is a toxic secondary metabolite produced by several Fusarium species that infest wheat and corn. Food and feed contaminated with DON pose a health risk to both humans and livestock and form a major barrier for international trade. Microbial detoxification represents an alternative approach to the physical and chemical detoxification methods of DON-contaminated grains. The present study details the characterization of a novel bacterium, Devosia mutans 17-2-E-8, that is capable of transforming DON to a non-toxic stereoisomer, 3-epi-deoxynivalenol under aerobic conditions, mild temperature (25-30°C), and neutral pH. The biotransformation takes place in the presence of rich sources of organic nitrogen and carbon without the need of DON to be the sole carbon source. The process is enzymatic in nature and endures a high detoxification capacity (3 μg DON/h/10(8) cells). The above conditions collectively suggest the possibility of utilizing the isolated bacterium as a feed treatment to address DON contamination under empirical field conditions.

Keywords: 3-epi-deoxynivalenol; Devosia; deoxynivalenol; epimerization; growth conditions; transformation

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