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Iqbal Z, Sattar MN, Shafiq M. CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease. Front Plant Sci. 2016;7:475doi: 10.3389/fpls.2016.00475.
Iqbal, Z., Sattar, M. N., & Shafiq, M. (2016). CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease. Frontiers in plant science, 7475. https://doi.org/10.3389/fpls.2016.00475
Iqbal, Zafar, et al. "CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease." Frontiers in plant science vol. 7 (2016): 475. doi: https://doi.org/10.3389/fpls.2016.00475
Iqbal Z, Sattar MN, Shafiq M. CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease. Front Plant Sci. 2016 Apr 12;7:475. doi: 10.3389/fpls.2016.00475. eCollection 2016. PMID: 27148303; PMCID: PMC4828465.
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Front Plant Sci. 2016 Apr 12;7:475. doi: 10.3389/fpls.2016.00475. eCollection 2016.

CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease.

Frontiers in plant science

Zafar Iqbal, Muhammad N Sattar, Muhammad Shafiq

Affiliations

  1. Institute of Biochemistry and Biotechnology, Quaid-i-Azam Campus, University of the Punjab Lahore, Pakistan.
  2. Department of Environment and Natural Resources, Faculty of Agriculture and Food Science, King Faisal University Al-Hasa, Saudi Arabia.
  3. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering Faisalabad, Pakistan.

PMID: 27148303 PMCID: PMC4828465 DOI: 10.3389/fpls.2016.00475

Abstract

The begomoviruses (family Geminiviridae) associated with cotton leaf curl disease (CLCuD) pose a major threat to cotton productivity in South-East Asia including Pakistan and India. These viruses have single-stranded, circular DNA genome, of ∼2800 nt in size, encapsidated in twinned icosa-hedera, transmitted by ubiquitous whitefly and are associated with satellite molecules referred to as alpha- and betasatellite. To circumvent the proliferation of these viruses numerous techniques, ranging from conventional breeding to molecular approaches have been applied. Such devised strategies worked perfectly well for a short time period and then viruses relapse due to various reasons including multiple infections, where related viruses synergistically interact with each other, virus proliferation and evolution. Another shortcoming is, until now, that all molecular biology approaches are devised to control only helper begomoviruses but not to control associated satellites. Despite the fact that satellites could add various functions to helper begomoviruses, they remain ignored. Such conditions necessitate a very comprehensive technique that can offer best controlling strategy not only against helper begomoviruses but also their associated DNA-satellites. In the current scenario clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) has proved to be versatile technique that has very recently been deployed successfully to control different geminiviruses. The CRISPR/Cas9 system has been proved to be a comprehensive technique to control different geminiviruses, however, like previously used techniques, only a single virus is targeted and hitherto it has not been deployed to control begomovirus complexes associated with DNA-satellites. Here in this article, we proposed an inimitable, unique, and broad spectrum controlling method based on multiplexed CRISPR/Cas9 system where a cassette of sgRNA is designed to target not only the whole CLCuD-associated begomovirus complex but also the associated satellite molecules.

Keywords: CRISPR/Cas9; alphasatellite; begomoviruses; betasatellites; cotton leaf curl disease

References

  1. Virus Res. 2000 Nov;71(1-2):151-9 - PubMed
  2. Virology. 2001 Jul 5;285(2):234-43 - PubMed
  3. Virology. 2001 Dec 5;291(1):110-25 - PubMed
  4. Arch Virol. 2003 Dec;148(12):2341-52 - PubMed
  5. J Virol. 2004 Jul;78(13):6758-65 - PubMed
  6. Virology. 2004 Jul 1;324(2):462-74 - PubMed
  7. J Virol. 2004 Sep;78(17):9487-98 - PubMed
  8. Arch Virol. 2004 Oct;149(10):2047-57 - PubMed
  9. Mol Plant Microbe Interact. 2005 Jan;18(1):7-14 - PubMed
  10. J Virol. 2005 Aug;79(15):9885-95 - PubMed
  11. J Virol. 2005 Aug;79(16):10764-75 - PubMed
  12. Microbiology. 2005 Aug;151(Pt 8):2551-61 - PubMed
  13. Arch Virol. 2007;152(7):1323-39 - PubMed
  14. Virus Res. 2007 Sep;128(1-2):135-9 - PubMed
  15. J Virol. 2007 Nov;81(21):11972-81 - PubMed
  16. J Gen Virol. 2007 Oct;88(Pt 10):2881-9 - PubMed
  17. Mol Plant Microbe Interact. 2007 Dec;20(12):1581-8 - PubMed
  18. Arch Virol. 2008;153(7):1367-72 - PubMed
  19. PLoS One. 2008;3(11):e3647 - PubMed
  20. Arch Virol. 2009;154(3):399-407 - PubMed
  21. Nat Biotechnol. 2009 Dec;27(12):1086-8; author reply 1088-9 - PubMed
  22. Virus Genes. 2010 Apr;40(2):282-9 - PubMed
  23. Mol Plant Pathol. 2010 Mar;11(2):245-54 - PubMed
  24. Mol Plant Pathol. 2004 Mar 1;5(2):149-56 - PubMed
  25. Mol Plant Pathol. 2003 Nov 1;4(6):427-34 - PubMed
  26. Virology. 2011 Jan 20;409(2):156-62 - PubMed
  27. Virol J. 2011 Mar 16;8:122 - PubMed
  28. Virol J. 2011 May 19;8:238 - PubMed
  29. Genet Mol Res. 2011 Oct 07;10(4):2404-14 - PubMed
  30. Annu Rev Genet. 2011;45:273-97 - PubMed
  31. Arch Virol. 2012 Mar;157(3):483-95 - PubMed
  32. Mol Ecol. 2012 Mar;21(5):1294-304 - PubMed
  33. Arch Virol. 2012 May;157(5):855-68 - PubMed
  34. Science. 2012 Aug 17;337(6096):816-21 - PubMed
  35. Virus Res. 2012 Oct;169(1):107-16 - PubMed
  36. PLoS One. 2012;7(8):e42168 - PubMed
  37. Science. 2013 Feb 15;339(6121):819-23 - PubMed
  38. J Gen Virol. 2013 Apr;94(Pt 4):695-710 - PubMed
  39. Nat Biotechnol. 2013 Mar;31(3):233-9 - PubMed
  40. Virus Genes. 2013 Jun;46(3):496-504 - PubMed
  41. Virol J. 2013 Jul 12;10:231 - PubMed
  42. Nat Biotechnol. 2013 Sep;31(9):827-32 - PubMed
  43. Nat Biotechnol. 2013 Sep;31(9):833-8 - PubMed
  44. Nat Biotechnol. 2013 Aug;31(8):688-91 - PubMed
  45. Cell Res. 2013 Oct;23(10):1229-32 - PubMed
  46. Cell. 2013 Sep 12;154(6):1380-9 - PubMed
  47. Bioinformatics. 2014 Apr 15;30(8):1180-1182 - PubMed
  48. Nat Methods. 2014 Feb;11(2):122-3 - PubMed
  49. Mol Plant. 2014 May;7(5):923-6 - PubMed
  50. Nat Biotechnol. 2014 Jun;32(6):577-582 - PubMed
  51. Nat Biotechnol. 2014 Jun;32(6):569-76 - PubMed
  52. Arch Virol. 2014 Oct;159(10):2787-90 - PubMed
  53. Nucleic Acids Res. 2014 Jul;42(Web Server issue):W401-7 - PubMed
  54. Sci Rep. 2014 Jun 23;4:5400 - PubMed
  55. Nucleic Acids Res. 2014 Oct 29;42(19):e147 - PubMed
  56. Nat Biotechnol. 2014 Dec;32(12):1262-7 - PubMed
  57. BMC Plant Biol. 2014 Nov 29;14:327 - PubMed
  58. ACS Synth Biol. 2015 Jun 19;4(6):723-8 - PubMed
  59. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5 - PubMed
  60. Anal Biochem. 2015 Jun 1;478:131-3 - PubMed
  61. Arch Virol. 2015 May;160(5):1219-28 - PubMed
  62. Arch Virol. 2015 Jun;160(6):1593-619 - PubMed
  63. Front Plant Sci. 2015 Jun 08;6:379 - PubMed
  64. Nat Rev Mol Cell Biol. 2015 Nov;16(11):642 - PubMed
  65. Genome Biol. 2015 Nov 11;16:238 - PubMed
  66. Science. 2016 Jan 1;351(6268):84-8 - PubMed
  67. Nature. 2016 Jan 28;529(7587):490-5 - PubMed
  68. Biochem Pharmacol. 2016 Apr 1;105:80-90 - PubMed
  69. Saudi J Biol Sci. 2016 May;23(3):358-62 - PubMed
  70. Nat Plants. 2015 Sep 28;1:15144 - PubMed
  71. Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 - PubMed
  72. J Gen Virol. 1998 Apr;79 ( Pt 4):915-23 - PubMed
  73. J Gen Virol. 1998 Jun;79 ( Pt 6):1501-8 - PubMed

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