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Kwon HO, Lee M, Kim OK, et al. Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models. Nutr Res Pract. 2016;10(3):265-73doi: 10.4162/nrp.2016.10.3.265.
Kwon, H. O., Lee, M., Kim, O. K., Ha, Y., Jun, W., & Lee, J. (2016). Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models. Nutrition research and practice, 10(3), 265-73. https://doi.org/10.4162/nrp.2016.10.3.265
Kwon, Han Ol, et al. "Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models." Nutrition research and practice vol. 10,3 (2016): 265-73. doi: https://doi.org/10.4162/nrp.2016.10.3.265
Kwon HO, Lee M, Kim OK, Ha Y, Jun W, Lee J. Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models. Nutr Res Pract. 2016 Jun;10(3):265-73. doi: 10.4162/nrp.2016.10.3.265. Epub 2016 Apr 01. PMID: 27247722; PMCID: PMC4880725.
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Nutr Res Pract. 2016 Jun;10(3):265-73. doi: 10.4162/nrp.2016.10.3.265. Epub 2016 Apr 01.

Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models.

Nutrition research and practice

Han Ol Kwon, Minhee Lee, Ok-Kyung Kim, Yejin Ha, Woojin Jun, Jeongmin Lee

Affiliations

  1. Department of Medical Nutrition, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin 17104, Korea.
  2. Division of Food and Nutritional Science, Chonnam National University, Gwangju 61186, Korea.

PMID: 27247722 PMCID: PMC4880725 DOI: 10.4162/nrp.2016.10.3.265

Abstract

BACKGROUND/OBJECTIVES: The inhibitory effect of Hijikia fusiforme (HF) extracts on degenerative osteoarthritis was examined in primary cultured rat cartilage cells and a monosodium iodoacetate (MIA)-induced osteoarthritis rat model.

MATERIALS/METHODS: In vitro, cell survival and the expression of matrix metalloproteinases (MMPs), collagen type I, collagen type II, aggrecan, and tissue inhibitor of metalloproteinases (TIMPs) was measured after H2O2 (800 µM, 2 hr) treatment in primary chondrocytes. In vivo animal study, osteoarthritis was induced by intra-articular injection of MIA into knee joints of rats, and then RH500, HFE250 and HFE500 were administered orally once a day for 28 days. To determine the anti-inflammatory effects of HFE, nitric oxide (NO), prostaglandin E2 (PGE2) expression were measured. In addition, real-time PCR was performed to measure the genetic expression of MMPs, collagen type I, collagen type II, aggrecan, and TIMPs.

RESULTS: In the in vitro assay, cell survival after H2O2 treatment was increased by HFE extract (20% EtOH). In addition, anabolic factors (genetic expression of collagen type I, II, and aggrecan) were increased by HFE extract (20% EtOH). However, the genetic expression of MMP-3 and 7, known as catabolic factors were significantly inhibited by treatment with HFE extract (20% EtOH). In the in vivo assay, anabolic factors (genetic expression of collagen type I, II, aggrecan, and TIMPs) were increased by oral administration of HFE extract. However, the genetic expression of MMP-3 and 7, known as catabolic factors, and production of NO and PGE2 were significantly inhibited by treatment with oral administration of HFE extract.

CONCLUSIONS: HFE extract inhibited articular cartilage degeneration through preventing extracellular matrix degradation and chondrocyte injury.

Keywords: Chondrocyte; Collagen; MMP; TIMP

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