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Li M, Yuan Y, Chen Q, et al. Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model. J Ophthalmol. 2016;2016:5126560doi: 10.1155/2016/5126560.
Li, M., Yuan, Y., Chen, Q., Me, R., Gu, Q., Yu, Y., Sheng, M., & Ke, B. (2016). Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model. Journal of ophthalmology, 20165126560. https://doi.org/10.1155/2016/5126560
Li, Min, et al. "Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model." Journal of ophthalmology vol. 2016 (2016): 5126560. doi: https://doi.org/10.1155/2016/5126560
Li M, Yuan Y, Chen Q, Me R, Gu Q, Yu Y, Sheng M, Ke B. Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model. J Ophthalmol. 2016;2016:5126560. doi: 10.1155/2016/5126560. Epub 2016 May 10. PMID: 27247798; PMCID: PMC4877496.
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J Ophthalmol. 2016;2016:5126560. doi: 10.1155/2016/5126560. Epub 2016 May 10.

Expression of Wnt/β-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-β1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model.

Journal of ophthalmology

Min Li, Ying Yuan, Qingzhong Chen, Rao Me, Qing Gu, Yunjie Yu, Minjie Sheng, Bilian Ke

Affiliations

  1. Department of Ophthalmology, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  2. Department of Ophthalmology, Yangpu Hospital, Tongji University, Shanghai, China.

PMID: 27247798 PMCID: PMC4877496 DOI: 10.1155/2016/5126560

Abstract

Background. To investigate Wnt/β-catenin signaling pathway expression and its regulation of type I collagen by TGF-β1 in scleral fibroblasts from form-deprivation myopia (FDM) guinea pig model. Methods. Wnt isoforms were examined using genome microarrays. Scleral fibroblasts from FDM group and self-control (SC) group were cultured. Wnt isoforms, β-catenin, TGF-β1, and type I collagen expression levels were examined in the two groups with or without DKK-1 or TGF-β1 neutralizing antibody. Results. For genome microarrays, the expression of Wnt3 in FDM group was significantly greater as confirmed in retinal and scleral tissue. The expression of Wnt3 and β-catenin significantly increased in FDM group and decreased significantly with DKK-1. TGF-β1 expression level decreased significantly in FDM group and increased significantly with DKK-1. Along with morphological misalignment inside and outside cells, the amount of type I collagen decreased in FDM group. Furthermore, type I collagen increased and became regular in DKK-1 intervention group, whereas it decreased and rearranged more disorder in TGF-β1 neutralizing antibody intervention group. Conclusions. The activation of Wnt3/β-catenin signaling pathway was demonstrated in primary scleral fibroblasts in FDM. This pathway further reduced the expression of type I collagen by TGF-β1, which ultimately played a role in scleral remodeling during myopia development.

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