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Trivedi AB, Doi E, Kitabatake N. Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions. Biosci Biotechnol Biochem. 1992;56(5):741-5doi: 10.1271/bbb.56.741.
Trivedi, A. B., Doi, E., & Kitabatake, N. (1992). Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions. Bioscience, biotechnology, and biochemistry, 56(5), 741-5. https://doi.org/10.1271/bbb.56.741
Trivedi, A B, et al. "Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions." Bioscience, biotechnology, and biochemistry vol. 56,5 (1992): 741-5. doi: https://doi.org/10.1271/bbb.56.741
Trivedi AB, Doi E, Kitabatake N. Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions. Biosci Biotechnol Biochem. 1992 Jan;56(5):741-5. doi: 10.1271/bbb.56.741. PMID: 27286200.
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Biosci Biotechnol Biochem. 1992 Jan;56(5):741-5. doi: 10.1271/bbb.56.741.

Detoxification of Ochratoxin A on Heating under Acidic and Alkaline Conditions.

Bioscience, biotechnology, and biochemistry

A B Trivedi, E Doi, N Kitabatake

Affiliations

  1. a Research Institute for Food Science, Kyoto University , Uji , Kyoto 611 , Japan.

PMID: 27286200 DOI: 10.1271/bbb.56.741

Abstract

Ochratoxin A was heated under three different moisture conditions, and under acidic and alkaline conditions. Heating to 175°C under the dry conditions produced little change in the molecule and cytotoxicity, detected by TLC and in the effects on the proliferation of HeLa cells. When heated under the moist and watery conditions, a small change in molecule was found by TLC, but cytotoxicity was not reduced. Under the acidic conditions (0.1 N HCl) the decomposition of ochratoxin A was detected by TLC, however the change in cytotoxicity was not observed in this assay system. On the other hand, heating with NaOH (0.1 N) resulted in the decomposition and detoxification of ochratoxin A. The HPTLC analysis showed the formation of some decomposed compounds including L-phenylalanine. This indicates the hydrolysis of ochratoxin A is one of the decomposition reactions induced by alkali at high temperatures.

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