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Rodríguez J, Mosquera J, García-Fandiño R, et al. A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves. Chem Sci. 2016;7(5):3298-3303doi: 10.1039/C6SC00045B.
Rodríguez, J., Mosquera, J., García-Fandiño, R., Vázquez, M. E., & Mascareñas, J. L. (2016). A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves. Chemical science, 7(5), 3298-3303. https://doi.org/10.1039/C6SC00045B
Rodríguez, Jéssica, et al. "A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves." Chemical science vol. 7,5 (2016): 3298-3303. doi: https://doi.org/10.1039/C6SC00045B
Rodríguez J, Mosquera J, García-Fandiño R, Vázquez ME, Mascareñas JL. A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves. Chem Sci. 2016 May 01;7(5):3298-3303. doi: 10.1039/C6SC00045B. Epub 2016 Feb 05. PMID: 27252825; PMCID: PMC4885664.
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Chem Sci. 2016 May 01;7(5):3298-3303. doi: 10.1039/C6SC00045B. Epub 2016 Feb 05.

A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves.

Chemical science

Jéssica Rodríguez, Jesús Mosquera, Rebeca García-Fandiño, M Eugenio Vázquez, José L Mascareñas

Affiliations

  1. Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

PMID: 27252825 PMCID: PMC4885664 DOI: 10.1039/C6SC00045B

Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

References

  1. ACS Chem Biol. 2012 Jun 15;7(6):1084-94 - PubMed
  2. Mol Cell. 2001 Nov;8(5):937-46 - PubMed
  3. J Am Chem Soc. 2003 Mar 26;125(12):3416-7 - PubMed
  4. Curr Opin Genet Dev. 1995 Apr;5(2):180-9 - PubMed
  5. Chem Commun (Camb). 2015 May 11;51(38):8130-3 - PubMed
  6. Biochemistry. 1992 Aug 4;31(30):6871-5 - PubMed
  7. Biochemistry. 2002 Feb 19;41(7):2177-83 - PubMed
  8. Angew Chem Int Ed Engl. 2012 Aug 27;51(35):8825-9 - PubMed
  9. Oncogene. 2001 May 28;20(24):3116-27 - PubMed
  10. Cell. 1992 Dec 24;71(7):1223-37 - PubMed
  11. Chem Biol. 2003 Aug;10(8):713-22 - PubMed
  12. Adv Drug Deliv Rev. 2005 Feb 28;57(4):637-51 - PubMed
  13. Chem Biol. 1998 Jun;5(6):R129-45 - PubMed
  14. Nat Commun. 2013;4:1874 - PubMed
  15. Chem Sci. 2015 Aug 1;6(8):4767-4771 - PubMed
  16. Chem Soc Rev. 2003 Nov;32(6):338-49 - PubMed
  17. Curr Opin Chem Biol. 2000 Feb;4(1):34-9 - PubMed
  18. Chem Soc Rev. 2011 Aug;40(8):4286-94 - PubMed
  19. Chemistry. 2013 Jul 22;19(30):9923-9 - PubMed
  20. Chembiochem. 2011 Sep 5;12(13):1958-73 - PubMed
  21. Chem Biol. 1996 May;3(5):369-77 - PubMed
  22. Curr Opin Struct Biol. 1999 Feb;9(1):48-55 - PubMed
  23. Angew Chem Int Ed Engl. 2001 Dec 17;40(24):4723-4725 - PubMed
  24. Chembiochem. 2005 Dec;6(12):2173-6 - PubMed
  25. Chem Commun (Camb). 2014 Mar 4;50(18):2258-60 - PubMed
  26. Org Biomol Chem. 2003 Jul 7;1(13):2217-20 - PubMed
  27. Annu Rev Biochem. 2010;79:213-31 - PubMed
  28. J Mol Biol. 1993 Sep 5;233(1):139-54 - PubMed
  29. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4841-5 - PubMed
  30. Nat Methods. 2014 Aug;11(8):861-7 - PubMed
  31. J Pept Res. 2000 May;55(5):398-408 - PubMed
  32. Curr Opin Chem Biol. 2005 Oct;9(5):467-74 - PubMed
  33. Chem Commun (Camb). 2015 Mar 21;51(23):4811-4 - PubMed
  34. Nature. 1991 Aug 8;352(6335):497-505 - PubMed
  35. Genome Biol. 2000;1(1):REVIEWS001 - PubMed
  36. Acc Chem Res. 2014 Aug 19;47(8):2309-18 - PubMed
  37. Nat Struct Biol. 1997 Aug;4(8):657-65 - PubMed
  38. J Am Chem Soc. 2003 Nov 5;125(44):13322-3 - PubMed
  39. Science. 1990 Aug 17;249(4970):769-71 - PubMed
  40. Biochimie. 2008 Jul;90(7):1015-25 - PubMed
  41. Curr Opin Struct Biol. 2003 Jun;13(3):284-99 - PubMed
  42. Drug Discov Today. 2012 Aug;17(15-16):850-60 - PubMed
  43. Org Biomol Chem. 2015 May 14;13(18):5273-8 - PubMed
  44. Chembiochem. 2008 Nov 24;9(17):2822-9 - PubMed
  45. Angew Chem Int Ed Engl. 2014 Sep 8;53(37):9917-21 - PubMed
  46. Chemistry. 2005 Jul 4;11(14):4171-8 - PubMed
  47. PLoS One. 2012;7(5):e37120 - PubMed
  48. Org Biomol Chem. 2015 Apr 7;13(13):3856-62 - PubMed
  49. Pharmaceuticals (Basel). 2012 Nov 01;5(11):1177-209 - PubMed
  50. Nat Biotechnol. 2002 Jul;20(7):729-33 - PubMed
  51. Angew Chem Int Ed Engl. 2007;46(36):6886-90 - PubMed
  52. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6981-5 - PubMed

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