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Biosci Biotechnol Biochem. 1998;62(12):2415-7. doi: 10.1271/bbb.62.2415.

Transglycosylation of Thiamin by Fungal β-N-Acetylhexosaminidases.

Bioscience, biotechnology, and biochemistry

V Kren, Z Hunkova, P Halada, Y Suzuki

Affiliations

  1. a Institute of Microbiology, Laboratory of Biotransformation, Academy of Sciences of the Czech Republic.
  2. b Research Institute for Bioresources, Okayama University.

PMID: 27392399 DOI: 10.1271/bbb.62.2415

Abstract

A new thiamin glycoside, e.g., thiamin β-D-2-deoxy-2-acetamidoglucopyranoside was prepared by transglycosylation by β-N-acetylhexosaminidase from Aspergillus oryzae and characterized spectrally. Series of other fungal β-N-acetyl-hexosaminidases from A. awamori, A. tamari, A. terreus, and Penicillium oxalicum were shown to be able to synthesize the same compound in lower yields.

Keywords: Aspergillus oryzae; thiamin; transglycosylation; vitamin; β-N-acetylhexosaminidases

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