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Liu L, Fujii T, Hayakawa K, et al. Prevention of Initial Supercooling in Progressive Freeze-concentration. Biosci Biotechnol Biochem. 1998;62(12):2467-9doi: 10.1271/bbb.62.2467.
Liu, L., Fujii, T., Hayakawa, K., & Miyawaki, O. (1998). Prevention of Initial Supercooling in Progressive Freeze-concentration. Bioscience, biotechnology, and biochemistry, 62(12), 2467-9. https://doi.org/10.1271/bbb.62.2467
Liu, L, et al. "Prevention of Initial Supercooling in Progressive Freeze-concentration." Bioscience, biotechnology, and biochemistry vol. 62,12 (1998): 2467-9. doi: https://doi.org/10.1271/bbb.62.2467
Liu L, Fujii T, Hayakawa K, Miyawaki O. Prevention of Initial Supercooling in Progressive Freeze-concentration. Biosci Biotechnol Biochem. 1998;62(12):2467-9. doi: 10.1271/bbb.62.2467. PMID: 27392406.
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Biosci Biotechnol Biochem. 1998;62(12):2467-9. doi: 10.1271/bbb.62.2467.

Prevention of Initial Supercooling in Progressive Freeze-concentration.

Bioscience, biotechnology, and biochemistry

L Liu, T Fujii, K Hayakawa, O Miyawaki

Affiliations

  1. a Department of Applied Biological Chemistry, The University of Tokyo.
  2. b Research Institute, Kagome Co. Ltd.

PMID: 27392406 DOI: 10.1271/bbb.62.2467

Abstract

A physical method is proposed that uses a cooling plate with many small holes to prevent initial supercooling in progressive freeze-concentration, and thus avoid serious contamination of the ice produced. The higher chance for ice nucleation of the water molecules in the holes due to the temperature gradient in the cooling plate resulted in the initial supercooling being completely prevented. Accordingly, the purity of the ice initially formed was substantially improved when compared with that by the standard vessel without holes in the cooling plate.

Keywords: freeze concentration; progressive freezing; purity of ice; supercooling

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