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Hirata K, Aso Y, Yasuda S, et al. Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities. Biosci Biotechnol Biochem. 1998;62(4):807-10doi: 10.1271/bbb.62.807.
Hirata, K., Aso, Y., Yasuda, S., & Ishiguro, M. (1998). Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities. Bioscience, biotechnology, and biochemistry, 62(4), 807-10. https://doi.org/10.1271/bbb.62.807
Hirata, K, et al. "Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities." Bioscience, biotechnology, and biochemistry vol. 62,4 (1998): 807-10. doi: https://doi.org/10.1271/bbb.62.807
Hirata K, Aso Y, Yasuda S, Ishiguro M. Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities. Biosci Biotechnol Biochem. 1998;62(4):807-10. doi: 10.1271/bbb.62.807. PMID: 27392563.
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Biosci Biotechnol Biochem. 1998;62(4):807-10. doi: 10.1271/bbb.62.807.

Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities.

Bioscience, biotechnology, and biochemistry

K Hirata, Y Aso, S Yasuda, M Ishiguro

Affiliations

  1. a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.

PMID: 27392563 DOI: 10.1271/bbb.62.807

Abstract

Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of α-fucosidase were purified from the viscera of Pomacea canaliculata. The thermostability of F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction and pH stability.

Keywords: Pomacea canaliculata; apple snail; isoform; α-fucosidase

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