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Masui N, Kato C. New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure. Biosci Biotechnol Biochem. 1999;63(1):235-7doi: 10.1271/bbb.63.235.
Masui, N., & Kato, C. (1999). New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure. Bioscience, biotechnology, and biochemistry, 63(1), 235-7. https://doi.org/10.1271/bbb.63.235
Masui, N, and Kato, C. "New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure." Bioscience, biotechnology, and biochemistry vol. 63,1 (1999): 235-7. doi: https://doi.org/10.1271/bbb.63.235
Masui N, Kato C. New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure. Biosci Biotechnol Biochem. 1999;63(1):235-7. doi: 10.1271/bbb.63.235. PMID: 27392880.
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Biosci Biotechnol Biochem. 1999;63(1):235-7. doi: 10.1271/bbb.63.235.

New Method of Screening for Pressure-sensitive Mutants at High Hydrostatic Pressure.

Bioscience, biotechnology, and biochemistry

N Masui, C Kato

Affiliations

  1. a The DEEP STAR Group, Japan Marine Science and Technology Center.

PMID: 27392880 DOI: 10.1271/bbb.63.235

Abstract

We have developed an efficient method of screening to detect pressure-sensitive mutants of barophilic or barotolerant bacteria using conventional agar medium plates. By this new method, 75 colonies can be screened per plate under high hydrostatic pressure.

Keywords: agar plate; barophilic bacteria; deep-sea; high hydrostatic pressure; pressure-sensitive mutant

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