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Moustafa FE, Sobh MA, Abouelkheir M, et al. Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats. Int J Stem Cells. 2016;9(1):79-89doi: 10.15283/ijsc.2016.9.1.79.
Moustafa, F. E., Sobh, M. A., Abouelkheir, M., Khater, Y., Mahmoud, K., Saad, M. A., & Sobh, M. A. (2016). Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats. International journal of stem cells, 9(1), 79-89. https://doi.org/10.15283/ijsc.2016.9.1.79
Moustafa, Fatma E, et al. "Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats." International journal of stem cells vol. 9,1 (2016): 79-89. doi: https://doi.org/10.15283/ijsc.2016.9.1.79
Moustafa FE, Sobh MA, Abouelkheir M, Khater Y, Mahmoud K, Saad MA, Sobh MA. Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats. Int J Stem Cells. 2016 May 30;9(1):79-89. doi: 10.15283/ijsc.2016.9.1.79. PMID: 27426089; PMCID: PMC4961107.
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Int J Stem Cells. 2016 May 30;9(1):79-89. doi: 10.15283/ijsc.2016.9.1.79.

Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats.

International journal of stem cells

Fatma E Moustafa, Mohamed-A Sobh, Mohamed Abouelkheir, Youmna Khater, Khalid Mahmoud, Mohamed-Ahdy Saad, Mohamed A Sobh

Affiliations

  1. Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
  2. Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
  3. Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
  4. Mansoura Medical Experimental Research Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

PMID: 27426089 PMCID: PMC4961107 DOI: 10.15283/ijsc.2016.9.1.79

Abstract

BACKGROUND AND OBJECTIVES: Mesenchymal stem cells (MSCs) have been shown to ameliorate cisplatin-induced acute kidney injury (AKI). The present study compares the efficacy of different routes of MSCs administration on kidney damage and regeneration after cisplatin-induced AKI.

METHODS: A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 160 rats. MSCs (5×10⁶) were given by either intravenous, intra-arterial or kidney sub capsular injection one day after cisplatin injection. Suitable control groups were included. Rats were sacrificed at 4, 7, 11 and 30 days after cisplatin injection. Kidney function parameters, kidney tissue oxidative stress markers, and scoring for renal tissue injury, regeneration and chronicity were all determined.

RESULTS: MSCs by any routes were able to ameliorate kidney function deterioration and renal tissue damage induced by cisplatin. The overall results of the three routes were equal. Differences between the different routes in one parameter were transient and inconsistent with other parameters.

CONCLUSIONS: Changing the route of MSCs injection does not have a major influence on the outcome. Future evaluation should focus on differences between the routes of administration considering the long term safety.

Keywords: Acute kidney injury; Cisplatin; Mesenchymal stem cells; Routes of administration; Sub capsular

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