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Sun LP, Xu X, Hwang HH, et al. Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis. Food Nutr Res. 2016;60:30081doi: 10.3402/fnr.v60.30081.
Sun, L. P., Xu, X., Hwang, H. H., Wang, X., Su, K. Y., & Chen, Y. L. (2016). Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis. Food & nutrition research, 6030081. https://doi.org/10.3402/fnr.v60.30081
Sun, Li-Ping, et al. "Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis." Food & nutrition research vol. 60 (2016): 30081. doi: https://doi.org/10.3402/fnr.v60.30081
Sun LP, Xu X, Hwang HH, Wang X, Su KY, Chen YL. Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis. Food Nutr Res. 2016 Jun 20;60:30081. doi: 10.3402/fnr.v60.30081. eCollection 2016. PMID: 27329777; PMCID: PMC4916261.
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Food Nutr Res. 2016 Jun 20;60:30081. doi: 10.3402/fnr.v60.30081. eCollection 2016.

Dichloromethane extracts of propolis protect cell from oxygen-glucose deprivation-induced oxidative stress via reducing apoptosis.

Food & nutrition research

Li-Ping Sun, Xiang Xu, Hau-Hsuan Hwang, Xin Wang, Kang-Yi Su, Yi-Lin S Chen

Affiliations

  1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
  2. Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
  3. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan; [email protected].
  4. Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan; [email protected].

PMID: 27329777 PMCID: PMC4916261 DOI: 10.3402/fnr.v60.30081

Abstract

BACKGROUND: Bee propolis, a mixture of the secretion from bee tongue gland and wax gland, was collected from the tree bud and barked by bees. The components were rich in terpenes, phenolics, and flavonoids, and had anti-cancer, anti-bacterial, anti-inflammatory, hepatoprotective, and neuroprotection abilities. However, the potential anti-oxidative stress of propolis was not well documented. This study aimed to study the protective effect of propolis on high-incident nonfatal diseases, such as stroke and cerebral infarction caused by ischemia.

OBJECTIVE: Oxidative stress caused by acute stroke results in inflammation and injury followed by cell damage and apoptosis. Clarification of the anti-oxidative stress effect of propolis may contribute to stroke prevention and damage reduction.

DESIGN: Propolis was separated and purified into 70% ethanol and dichloromethane extracts systematically. The fraction three (Fr.3) of dichloromethane was further separated into pinocembrin, pinobanksin, pinobanksin-3-acetate, chrysin, and galangin by chromatography. Compounds extracted from propolis were tested for cell-protection effects in an oxygen-glucose deprivation (OGD) N2a cell model. MTT assay, oxidative stress markers measurement, flow cytometry, and QPCR were used to evaluate cell viability and apoptosis.

RESULTS: All compounds, especially pinocembrin and galangin, enhanced cell viability in OGD-treated N2a cells. In addition, anti-oxidative enzymes were elevated and cellular Ca(2+) was reduced. They also had extreme anti-apoptosis effects by up-regulating the expression of Bcl-2 mRNA and down-regulating caspase-3 and Bax expression. Taken together, propolis had anti-oxidative effects on stress and protected cells from damage.

CONCLUSION: The anti-oxidative effect of propolis can be applied to daily food supplements and may benefit stroke patients.

Keywords: OGD; anti-apoptosis; ischemia-reperfusion injury; oxidative stress; propolis

References

  1. J Agric Food Chem. 2012 Nov 28;60(47):11748-58 - PubMed
  2. Hum Exp Toxicol. 2011 Sep;30(9):1246-55 - PubMed
  3. J Ethnopharmacol. 2007 Sep 5;113(2):278-83 - PubMed
  4. J Agric Food Chem. 2014 Mar 27;:null - PubMed
  5. Pharmacol Ther. 2005 Mar;105(3):229-66 - PubMed
  6. J Pharmacol Sci. 2005 Sep;99(1):39-44 - PubMed
  7. Annu Rev Plant Biol. 2004;55:373-99 - PubMed
  8. Int J Stroke. 2009 Dec;4(6):461-70 - PubMed
  9. Evid Based Complement Alternat Med. 2012;2012:981896 - PubMed
  10. Nutr Res. 2012 Feb;32(2):133-43 - PubMed
  11. Evid Based Complement Alternat Med. 2005 Mar;2(1):85-92 - PubMed
  12. Antivir Chem Chemother. 2008;19(1):7-13 - PubMed
  13. Food Chem Toxicol. 1998 Apr;36(4):347-63 - PubMed
  14. Stroke. 2009 May;40(5):e331-9 - PubMed
  15. J Am Heart Assoc. 2015 Apr 10;4(4):null - PubMed
  16. J Ethnopharmacol. 2005 Sep 14;100(3):276-83 - PubMed
  17. Mol Neurobiol. 2013 Feb;47(1):9-23 - PubMed
  18. J Physiol. 2007 Jun 1;581(Pt 2):431-44 - PubMed
  19. FEBS J. 2005 Jul;272(14):3593-601 - PubMed
  20. Nat Prod Rep. 2011 May;28(5):925-36 - PubMed
  21. Stroke. 2005 Dec;36(12):2712-7 - PubMed
  22. Brain Res. 2008 Mar 27;1201:135-42 - PubMed
  23. Experientia. 1988 Mar 15;44(3):230-2 - PubMed
  24. CNS Neurol Disord Drug Targets. 2013 Aug;12(5):698-714 - PubMed
  25. Circ Res. 2004 Oct 1;95(7):692-9 - PubMed
  26. J Neurosci. 2000 Dec 1;20(23):8597-603 - PubMed
  27. Acta Pharm. 2004 Mar;54(1):65-72 - PubMed
  28. J Agric Food Chem. 2012 Dec 19;60(50):12403-10 - PubMed
  29. Cancer Lett. 2007 Jul 18;252(2):235-43 - PubMed
  30. J Agric Food Chem. 2008 Nov 12;56(21):9927-32 - PubMed
  31. J Appl Microbiol. 2007 Nov;103(5):1914-21 - PubMed
  32. Circulation. 2003 Mar 18;107(10):1424-32 - PubMed
  33. Ross Fiziol Zh Im I M Sechenova. 2000 Feb;86(2):148-63 - PubMed

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