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Hoshino T, Yamamoto M. Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum. Biosci Biotechnol Biochem. 1997;61(12):2134-6doi: 10.1271/bbb.61.2134.
Hoshino, T., & Yamamoto, M. (1997). Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum. Bioscience, biotechnology, and biochemistry, 61(12), 2134-6. https://doi.org/10.1271/bbb.61.2134
Hoshino, T, and Yamamoto, M. "Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum." Bioscience, biotechnology, and biochemistry vol. 61,12 (1997): 2134-6. doi: https://doi.org/10.1271/bbb.61.2134
Hoshino T, Yamamoto M. Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum. Biosci Biotechnol Biochem. 1997 Jan;61(12):2134-6. doi: 10.1271/bbb.61.2134. PMID: 27396892.
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Biosci Biotechnol Biochem. 1997 Jan;61(12):2134-6. doi: 10.1271/bbb.61.2134.

Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum.

Bioscience, biotechnology, and biochemistry

T Hoshino, M Yamamoto

Affiliations

  1. a Department of Applied Biological Chemistry, Faculty of Agriculture , Niigata University , Ikarashi, Niigata 950-21 , Japan.

PMID: 27396892 DOI: 10.1271/bbb.61.2134

Abstract

A cell-free system for converting tryptophan precursor into violacein pigments is reported. Crucial factors were the requirements of the reduced nicotinamides as a cofactor and Zn(2+) as a metal ion. Optimal pHs were in the range of 8.5-9.5. The effectiveness of NADH was thirty times lower than that of NADPH at a low concentration of 2mm. The oxygenation mechanism(s) is discussed by using oxygenase inhibitors such as amethopterin, metyrapone, ancymidol, and prohexadione. Metal-chelating agents strongly inhibited the biosynthesis, suggesting that metallo-enzymes are involved in the biosynthesis.

Keywords: NADPH; cell-free biosynthesis; metalio-enzyme; tryptophan; violacein

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