Display options
Cite
Kamarudin TA, Othman F, Mohd Ramli ES, et al. Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count. EXCLI J. 2012;11:226-36
Kamarudin, T. A., Othman, F., Mohd Ramli, E. S., Md Isa, N., & Das, S. (2012). Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count. EXCLI journal, 11226-36.
Kamarudin, Taty Anna, et al. "Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count." EXCLI journal vol. 11 (2012): 226-36.
Kamarudin TA, Othman F, Mohd Ramli ES, Md Isa N, Das S. Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count. EXCLI J. 2012 May 15;11:226-36. eCollection 2012. PMID: 27366139; PMCID: PMC4928023.
Copy Download .nbib Format: NLM
Share it on

EXCLI J. 2012 May 15;11:226-36. eCollection 2012.

Protective effect of curcumin on experimentally induced arthritic rats: detailed histopathological study of the joints and white blood cell count.

EXCLI journal

Taty Anna Kamarudin, Faizah Othman, Elvy Suhana Mohd Ramli, Nurismah Md Isa, Srijit Das

Affiliations

  1. Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 Kuala Lumpur, MALAYSIA.

PMID: 27366139 PMCID: PMC4928023

Abstract

Curcuma longa (turmeric) rhizomes contains curcumin, an active compound which possesses anti-inflammatory effects. Collagen-induced arthritis (CIA) is an accepted experimental animal model of rheumatoid arthritis. The present study aimed to observe the histological changes in the joints of experimental arthritic rats treated with curcumin. Twenty four male Sprague-Dawley (approximately 7 weeks-old) rats were randomly divided into four groups. Three groups were immunized with 150 µg collagen. All rats with established CIA, with arthritis scores exceeding 1, were orally treated with betamethasone (0.5 mg/ml/kg body weight), curcumin (110 mg/ml/kg body weight) or olive oil (1.0 ml/kg body weight) daily, for two weeks. One remaining group was kept as normal control. Treatment with 110 mg/ml/kg curcumin showed significant mean differences in the average white blood cell (WBC) count (p<0.05), cell infiltration, bone and cartilage erosion scores (p<0.05) compared to the olive oil treated group. Pannus formation scores showed that curcumin supplementation successfully suppressed the pannus formation process that occurred in the articular cartilage of the CIA joints. The mean difference for histological scores for the curcumin group was insignificant compared to the betamethasone treated group. It is concluded that supplementation of curcumin has protective effect on the histopathological and degenerative changes in the joints of CIA rats which was at par with betamethasone.

Keywords: arthritis; collagen; curcumin; experimental; histopathology; inflammation

References

  1. Rheumatol Int. 2006 Aug;26(10 ):908-15 - PubMed
  2. Int J Biochem Cell Biol. 2004 Mar;36(3):372-8 - PubMed
  3. Semin Arthritis Rheum. 2001 Apr;30(5 Suppl 2):1-6 - PubMed
  4. Arch Orthop Trauma Surg. 1998;117(1-2):2-7 - PubMed
  5. J Clin Immunol. 2007 Jan;27(1):19-35 - PubMed
  6. Inflammopharmacology. 2004;12(1):89-94 - PubMed
  7. Rheumatology (Oxford). 2004 Apr;43(4):428-34 - PubMed
  8. J Surg Res. 2004 Oct;121(2):171-7 - PubMed
  9. Inflamm Res. 2006 Apr;55(4):168-75 - PubMed
  10. Immunol Invest. 1999 Sep-Dec;28(5-6):291-303 - PubMed
  11. J Nat Prod. 2006 Mar;69(3):351-5 - PubMed
  12. Arthritis Res. 2002;4 Suppl 3:S81-90 - PubMed
  13. Clin Ter. 2011;162(3):201-7 - PubMed
  14. Crit Rev Food Sci Nutr. 2004;44(2):97-111 - PubMed
  15. Ann Rheum Dis. 2002 Nov;61 Suppl 2:ii84-6 - PubMed
  16. Arthritis Res Ther. 2009;11(1):210 - PubMed
  17. Biochem Pharmacol. 2007 May 1;73(9):1434-45 - PubMed
  18. Semin Arthritis Rheum. 2001 Apr;30(5 Suppl 2):7-16 - PubMed
  19. Nat Protoc. 2007;2(5):1269-75 - PubMed
  20. Adv Exp Med Biol. 2007;595:453-70 - PubMed
  21. Inflamm Res. 2005 May;54(5):211-20 - PubMed
  22. J Clin Invest. 1978 Jan;61(1):89-96 - PubMed
  23. Life Sci. 2006 Mar 27;78(18):2081-7 - PubMed
  24. Ann Rheum Dis. 1999 Nov;58 Suppl 1:I40-8 - PubMed
  25. Eur J Cancer. 2005 Sep;41(13):1955-68 - PubMed
  26. Immunol Lett. 2006 Jul 15;106(1):8-13 - PubMed
  27. Arthritis Res. 2002;4(5):R7 - PubMed
  28. Arthritis Rheum. 2007 Feb;56(2):544-54 - PubMed
  29. Adv Exp Med Biol. 2007;595:425-51 - PubMed
  30. J Pharmacol Exp Ther. 2008 Aug;326(2):532-45 - PubMed
  31. Semin Arthritis Rheum. 2001 Dec;31(3):160-75 - PubMed
  32. Biochem Biophys Res Commun. 2005 Nov 18;337(2):586-94 - PubMed
  33. Am J Pathol. 2002 Oct;161(4):1419-27 - PubMed
  34. Biosci Biotechnol Biochem. 2008 Jul;72(7):1781-8 - PubMed

Publication Types