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Venkatesan B, Tumala A, Subramanian V, et al. Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart. Data Brief. 2016;8:441-7doi: 10.1016/j.dib.2016.05.074.
Venkatesan, B., Tumala, A., Subramanian, V., & Vellaichamy, E. (2016). Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart. Data in brief, 8441-7. https://doi.org/10.1016/j.dib.2016.05.074
Venkatesan, Balaji, et al. "Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart." Data in brief vol. 8 (2016): 441-7. doi: https://doi.org/10.1016/j.dib.2016.05.074
Venkatesan B, Tumala A, Subramanian V, Vellaichamy E. Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart. Data Brief. 2016 Jun 03;8:441-7. doi: 10.1016/j.dib.2016.05.074. eCollection 2016 Sep. PMID: 27366782; PMCID: PMC4910299.
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Data Brief. 2016 Jun 03;8:441-7. doi: 10.1016/j.dib.2016.05.074. eCollection 2016 Sep.

Data on synthesis and characterization of chitosan nanoparticles for in vivo delivery of siRNA-Npr3: Targeting NPR-C expression in the heart.

Data in brief

Balaji Venkatesan, Anusha Tumala, Vimala Subramanian, Elangovan Vellaichamy

Affiliations

  1. Department of Biochemistry, University of Madras, Guindy campus, Chennai 600025, India.

PMID: 27366782 PMCID: PMC4910299 DOI: 10.1016/j.dib.2016.05.074

Abstract

This data article contains the data related to the research article 'Transient silencing of Npr3 gene expression improved the circulatory levels of atrial natriuretic peptides and attenuated β-adrenoceptor activation-induced cardiac hypertrophic growth in experimental rats' (Venkatesan et al., 2016 [1]). The siRNA-Npr3 loaded chitosan nanoparticles were synthesized using ionotropic gelation method, where the positive charge of the chitosan interacts with the negative charge of STPP and siRNA-Npr3. The physicochemical properties of the synthesized siRNA-Npr3 loaded chitosan nanoparticles were studied by dynamic light scattering, FE-SEM and HR-TEM analysis. In addition, the loading efficiency and stability of the nanoparticles were also studied. Further, the gene silencing efficacy, hemocompatibility and biocompatibility were studied using Wistar rats (in vivo), isolated red blood cells and H9c2 cardiomyoblast cells, respectively.

Keywords: Biocompatibility; Chitosan nanoparticles; Gene silencing; Hemocompatibility

References

  1. Eur J Pharmacol. 2016 Jul 5;782:44-58 - PubMed
  2. Mol Ther. 2006 Oct;14 (4):476-84 - PubMed
  3. J Immunol Methods. 1983 Dec 16;65(1-2):55-63 - PubMed
  4. J Control Release. 2006 Oct 10;115(2):216-25 - PubMed
  5. Asian Pac J Trop Med. 2014 Sep;7S1:S294-300 - PubMed

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