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Parasitol Int. 2016 Oct;65(5):599-602. doi: 10.1016/j.parint.2016.07.008. Epub 2016 Jul 16.

Laboratory propagation of freshwater cyclopoid copepods as an intermediate host for parasitic helminths.

Parasitology international

Hideo Kumazawa

Affiliations

  1. Department of Parasitology, Kochi Medical School, Nankoku, Kochi 783-8505, Japan. Electronic address: [email protected].

PMID: 27430368 DOI: 10.1016/j.parint.2016.07.008

Abstract

Among the freshwater cyclopoids, small and medium-sized species can be propagated in the laboratory with a flagellate Chilomonas as the food. By contrast, large species need to be given, in addition to the flagellate, a larger food organism. For this, although a metazoan food is preferable, a ciliate Stentor may in part serve as a substitute. Methods for infection experiment involving copepods are given, and photographs of some parasite larvae in the copepod host are shown. Usage of carbonated water is helpful to immobilize cyclopoids and thus recommended for infection experiments.

Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Keywords: Culture; Cyclopidae; Food organism; Infection experiment; Intermediate host

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