Parasitol Int. 2016 Oct;65(5):599-602. doi: 10.1016/j.parint.2016.07.008. Epub 2016 Jul 16.
Parasitology international
Hideo Kumazawa
PMID: 27430368 DOI: 10.1016/j.parint.2016.07.008
Among the freshwater cyclopoids, small and medium-sized species can be propagated in the laboratory with a flagellate Chilomonas as the food. By contrast, large species need to be given, in addition to the flagellate, a larger food organism. For this, although a metazoan food is preferable, a ciliate Stentor may in part serve as a substitute. Methods for infection experiment involving copepods are given, and photographs of some parasite larvae in the copepod host are shown. Usage of carbonated water is helpful to immobilize cyclopoids and thus recommended for infection experiments.
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Keywords: Culture; Cyclopidae; Food organism; Infection experiment; Intermediate host