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Yamaguchi F, Ogawa Y, Kikuchi M, et al. Detection of γ-Polyglutamic Acid (γ-PGA) by SDS-Page. Biosci Biotechnol Biochem. 1996;60(2):255-8doi: 10.1271/bbb.60.255.
Yamaguchi, F., Ogawa, Y., Kikuchi, M., Yuasa, K., & Motai, H. (1996). Detection of γ-Polyglutamic Acid (γ-PGA) by SDS-Page. Bioscience, biotechnology, and biochemistry, 60(2), 255-8. https://doi.org/10.1271/bbb.60.255
Yamaguchi, F, et al. "Detection of γ-Polyglutamic Acid (γ-PGA) by SDS-Page." Bioscience, biotechnology, and biochemistry vol. 60,2 (1996): 255-8. doi: https://doi.org/10.1271/bbb.60.255
Yamaguchi F, Ogawa Y, Kikuchi M, Yuasa K, Motai H. Detection of γ-Polyglutamic Acid (γ-PGA) by SDS-Page. Biosci Biotechnol Biochem. 1996 Jan;60(2):255-8. doi: 10.1271/bbb.60.255. PMID: 27299401.
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Biosci Biotechnol Biochem. 1996 Jan;60(2):255-8. doi: 10.1271/bbb.60.255.

Detection of γ-Polyglutamic Acid (γ-PGA) by SDS-Page.

Bioscience, biotechnology, and biochemistry

F Yamaguchi, Y Ogawa, M Kikuchi, K Yuasa, H Motai

Affiliations

  1. a Research and Development Division of Kikkoman Corporation , 399 Noda, Noda-shi, Chiba , 278 , Japan.

PMID: 27299401 DOI: 10.1271/bbb.60.255

Abstract

γ-Polyglutamic acid (γ-PGA) produced by Bacillus suhtilis (natto) was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and basic dye staining. Using this method, the molecular weight of γ-PGA was estimated at 275,000. This value was almost the same in all bacterial strains tested. As other applications of this SDS-PAGE method, degradation of γ-PGA by acid and heat treatment and a cross-linking reaction with carbodiimide and ethylenediamine were made visible in acrylamide gel. In the growth curve of the bacteria, γ-PGA production was detected in early stationary phase by SDS-PAGE.

Keywords: Bacillus suhtilis; basic dye; natto; polyacrylamide gel electrophoresis; γ-polyglutamic acid

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