Rev Sci Instrum. 2016 Jun;87(6):066103. doi: 10.1063/1.4952992.
The Review of scientific instruments
K E Jensen, N Nakamura
PMID: 27370504 DOI: 10.1063/1.4952992
Confocal microscopy of colloids combined with digital image processing has become a powerful tool in soft matter physics and materials science. Together, these techniques enable locating and tracking of more than half a million individual colloidal particles at once. However, despite improvements in locating algorithms that improve position accuracy, it remains challenging to locate all particles in a densely packed, three dimensional colloid without erroneously identifying the same particle more than once. We present a simple iterative algorithm that mitigates both the "missed particle" and "double counting" problems while simultaneously reducing sensitivity to the specific choice of input parameters. It is also useful for analyzing images with spatially varying brightness in which a single set of input parameters is not appropriate for all particles. The algorithm is easy to implement and compatible with existing particle locating software.
