Display options
Cite
Atabo S, Umar IA, James DB, et al. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica (Cairo). 2016;2016:1568061doi: 10.1155/2016/1568061.
Atabo, S., Umar, I. A., James, D. B., & Mamman, A. I. (2016). Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica, 20161568061. https://doi.org/10.1155/2016/1568061
Atabo, Samuel, et al. "Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis." Scientifica vol. 2016 (2016): 1568061. doi: https://doi.org/10.1155/2016/1568061
Atabo S, Umar IA, James DB, Mamman AI. Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis. Scientifica (Cairo). 2016;2016:1568061. doi: 10.1155/2016/1568061. Epub 2016 Jun 28. PMID: 27433373; PMCID: PMC4940565.
Copy Download .nbib Format: NLM
Share it on

Scientifica (Cairo). 2016;2016:1568061. doi: 10.1155/2016/1568061. Epub 2016 Jun 28.

Sickled Erythrocytes Reversal and Membrane Stabilizing Compounds in Telfairia occidentalis.

Scientifica

Samuel Atabo, Ismaila Alhaji Umar, Dorcas Bolanle James, Aisha Indo Mamman

Affiliations

  1. Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
  2. Department of Hematology, Ahmadu Bello University Teaching Hospital, Shika, Zaria, Nigeria.

PMID: 27433373 PMCID: PMC4940565 DOI: 10.1155/2016/1568061

Abstract

Background and Purpose. Traditional management of sickle cell disease (SCD) is ubiquitous in Africa. In south-eastern Nigeria, Telfairia occidentalis (T. occidentalis) is strongly recommended for consumption by SCD patients, owing to its presumed therapeutic effect. This study investigates the antisickling and membrane regenerative potentials of T. occidentalis in sickled erythrocytes. Experimental Approach. Sickled erythrocytes obtained from SCD patients were treated with sodium metabisulphite (2%) to induce further sickling. Heat and hypotonic-induced lyses of red blood cells' membranes were also carried out. The RBCs were treated with varying concentration (10.0, 1.0, and 0.1 mg mL(-1) and 0.5, 1.0, 1.5, 2.0, and 2.5 mg mL(-1), resp.) of T. occidentalis extracts as treatment regimen for in vitro antisickling and membrane stabilizing assays. Extract with peak activity was purified and reused in antisickling assay. Key Results. The antisickling activity of aqueous and methanolic extracts of leaves, seeds, and stem of Telfairia occidentalis at 10.0, 1.0, and 0.1 mg mL(-1) revealed that the aqueous leaves extract (10 mg mL(-1)) exhibited the highest antisickling activity (64.03%) which was significantly (p < 0.05) higher than that of the stem (47.30%) and seeds (37.50%). Partially purified fractions recorded improved antisickling effect (peak activity of 70%). Characterization (using GC-MS) of the most active fraction revealed some bioactive compounds. In the membrane stabilizing assay, methanolic and aqueous stem extracts of T. occidentalis showed the highest effect of 71.85% and 61.29%, respectively. Conclusions and Implications. The results provide scientific evidence for ethnopharmacological use of T. occidentalis in the management of SCD.

References

  1. PLoS Med. 2013;10(7):e1001484 - PubMed
  2. J Trop Pediatr. 2005 Aug;51(4):200-5 - PubMed
  3. Lloydia. 1971 Dec;34(4):383-5 - PubMed
  4. PLoS Negl Trop Dis. 2013 May 30;7(5):e2120 - PubMed
  5. Blood Transfus. 2010 Oct;8(4):248-54 - PubMed
  6. Trans R Soc Trop Med Hyg. 1987;81(3):510-1 - PubMed
  7. Planta Med. 1990 Feb;56(1):41-3 - PubMed
  8. J Ethnopharmacol. 2005 Oct 31;102(1):15-22 - PubMed
  9. In Vivo. 2013 Nov-Dec;27(6):809-14 - PubMed
  10. Curr Med Chem. 2001 Feb;8(2):79-88 - PubMed
  11. Chem Biodivers. 2012 Aug;9(8):1525-32 - PubMed

Publication Types