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Zolt SD, Thermann R, Bangsow T, et al. Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres. Transfus Med Hemother. 2016;43(3):183-9doi: 10.1159/000446290.
Zolt, S. D., Thermann, R., Bangsow, T., Pichl, L., Müller, B., Jork, C., Weber-Schehl, M., Hedges, D., Schupp, I., Unverzagt, P., de Rue, K., & Roth, W. K. (2016). Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres. Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 43(3), 183-9. https://doi.org/10.1159/000446290
Zolt, Silke De, et al. "Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres." Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie vol. 43,3 (2016): 183-9. doi: https://doi.org/10.1159/000446290
Zolt SD, Thermann R, Bangsow T, Pichl L, Müller B, Jork C, Weber-Schehl M, Hedges D, Schupp I, Unverzagt P, de Rue K, Roth WK. Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres. Transfus Med Hemother. 2016 May;43(3):183-9. doi: 10.1159/000446290. Epub 2016 May 11. PMID: 27403090; PMCID: PMC4924456.
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Transfus Med Hemother. 2016 May;43(3):183-9. doi: 10.1159/000446290. Epub 2016 May 11.

Implementation of an HIV-1 Triple-Target NAT Assay in the Routine Screening at Three German Red Cross Blood Centres.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Silke De Zolt, Rolf Thermann, Thorsten Bangsow, Lutz Pichl, Benjamin Müller, Christine Jork, Marijke Weber-Schehl, Doris Hedges, Ingo Schupp, Patrick Unverzagt, Katrin de Rue, W Kurt Roth

Affiliations

  1. Gesellschaft zur Forschung, Entwicklung und Distribution von Diagnostika im Blutspendewesen mbH (GFE Blut), Frankfurt/M., Germany.
  2. DRK Blutspendedienst West, Hagen, Germany.
  3. DRK Blutspendedienst NSTOB, Springe, Germany.
  4. Blutspendedienst des Bayerischen Roten Kreuzes, Wiesentheid, Germany.

PMID: 27403090 PMCID: PMC4924456 DOI: 10.1159/000446290

Abstract

BACKGROUND: Blood product safety was significantly improved by the introduction of NAT testing in the late 1990s, resulting in a strong decrease of transfusion-transmitted infections (TTIs). Due to the occurrence of HIV-1 NAT test failures as a consequence of mismatch mutations in the amplicon regions of mono-target NAT assays, the Paul Ehrlich Institute mandated the implementation of multi-target NAT assays for HIV-1 in 2014. Commercial suppliers mostly developed dual-target NAT assays, with only one implementing a triple-target NAT assay.

METHODS: The HIV-1 triple-target NAT assay v3 (GFE Blut) was tested on mutated specimens and synthetic DNA bearing mutations that resulted in sample underquantification or false-negative test results. In addition, data from 2 years routine testing at three German Red Cross Blood centres were analysed.

RESULTS: The HIV-1 triple-target PCR could compensate for all mutations tested and could compensate the loss of one amplicon without a significant loss of sensitivity. Data from 2 years routine testing showed a solid performance.

CONCLUSION: The HIV-1 triple-target v3 assay (GFE Blut) can compensate mutations in target sequences better than a dual-target assay and is applicable to high-throughput screening, thus increasing blood product safety.

Keywords: Blood safety; HIV-1 triple-target NAT; HIV-1 variants; High throughput testing; Multi-target PCR; NAT

References

  1. Bioinformatics. 2009 May 1;25(9):1189-91 - PubMed
  2. Transfus Med Hemother. 2014 Feb;41(1):45-51 - PubMed
  3. Transfusion. 2011 Apr;51(4):719-30 - PubMed
  4. Transfusion. 2009 Sep;49(9):1850-8 - PubMed
  5. Transfusion. 2010 Mar;50(3):685-92 - PubMed
  6. Mol Biol Evol. 2013 Dec;30(12):2725-9 - PubMed
  7. Transfus Med. 2002 Aug;12(4):255-8 - PubMed
  8. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
  9. J Med Virol. 1994 May;43(1):72-6 - PubMed
  10. Transfusion. 2013 Oct;53(10 Pt 2):2422-30 - PubMed
  11. Transfusion. 2009 Sep;49(9):1836-44 - PubMed
  12. Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W463-5 - PubMed
  13. Transfusion. 2008 Aug;48(8):1558-66 - PubMed
  14. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2012 Aug;55(8):907-13 - PubMed
  15. Transfusion. 2012 Feb;52(2):431-9 - PubMed
  16. Lancet. 1999 Jan 30;353(9150):359-63 - PubMed
  17. Vox Sang. 2012 Jan;102(1):82-90 - PubMed

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