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Labeeuw L, Khey J, Bramucci AR, et al. Indole-3-Acetic Acid Is Produced by Emiliania huxleyi Coccolith-Bearing Cells and Triggers a Physiological Response in Bald Cells. Front Microbiol. 2016;7:828doi: 10.3389/fmicb.2016.00828.
Labeeuw, L., Khey, J., Bramucci, A. R., Atwal, H., de la Mata, A. P., Harynuk, J., & Case, R. J. (2016). Indole-3-Acetic Acid Is Produced by Emiliania huxleyi Coccolith-Bearing Cells and Triggers a Physiological Response in Bald Cells. Frontiers in microbiology, 7828. https://doi.org/10.3389/fmicb.2016.00828
Labeeuw, Leen, et al. "Indole-3-Acetic Acid Is Produced by Emiliania huxleyi Coccolith-Bearing Cells and Triggers a Physiological Response in Bald Cells." Frontiers in microbiology vol. 7 (2016): 828. doi: https://doi.org/10.3389/fmicb.2016.00828
Labeeuw L, Khey J, Bramucci AR, Atwal H, de la Mata AP, Harynuk J, Case RJ. Indole-3-Acetic Acid Is Produced by Emiliania huxleyi Coccolith-Bearing Cells and Triggers a Physiological Response in Bald Cells. Front Microbiol. 2016 Jun 08;7:828. doi: 10.3389/fmicb.2016.00828. eCollection 2016. PMID: 27375567; PMCID: PMC4896954.
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Front Microbiol. 2016 Jun 08;7:828. doi: 10.3389/fmicb.2016.00828. eCollection 2016.

Indole-3-Acetic Acid Is Produced by Emiliania huxleyi Coccolith-Bearing Cells and Triggers a Physiological Response in Bald Cells.

Frontiers in microbiology

Leen Labeeuw, Joleen Khey, Anna R Bramucci, Harjot Atwal, A Paulina de la Mata, James Harynuk, Rebecca J Case

Affiliations

  1. Department of Biological Sciences, University of Alberta Edmonton, AB, Canada.
  2. Department of Chemistry, University of Alberta Edmonton, AB, Canada.

PMID: 27375567 PMCID: PMC4896954 DOI: 10.3389/fmicb.2016.00828

Abstract

Indole-3-acetic acid (IAA) is an auxin produced by terrestrial plants which influences development through a variety of cellular mechanisms, such as altering cell orientation, organ development, fertility, and cell elongation. IAA is also produced by bacterial pathogens and symbionts of plants and algae, allowing them to manipulate growth and development of their host. They do so by either producing excess exogenous IAA or hijacking the IAA biosynthesis pathway of their host. The endogenous production of IAA by algae remains contentious. Using Emiliania huxleyi, a globally abundant marine haptophyte, we investigated the presence and potential role of IAA in algae. Homologs of genes involved in several tryptophan-dependent IAA biosynthesis pathways were identified in E. huxleyi. This suggests that this haptophyte can synthesize IAA using various precursors derived from tryptophan. Addition of L-tryptophan to E. huxleyi stimulated IAA production, which could be detected using Salkowski's reagent and GC × GC-TOFMS in the C cell type (coccolith bearing), but not in the N cell type (bald). Various concentrations of IAA were exogenously added to these two cell types to identify a physiological response in E. huxleyi. The N cell type, which did not produce IAA, was more sensitive to it, showing an increased variation in cell size, membrane permeability, and a corresponding increase in the photosynthetic potential quantum yield of Photosystem II (PSII). A roseobacter (bacteria commonly associated with E. huxleyi) Ruegeria sp. R11, previously shown to produce IAA, was co-cultured with E. huxleyi C and N cells. IAA could not be detected from these co-cultures, and even when stimulated by addition of L-tryptophan, they produced less IAA than axenic C type culture similarly induced. This suggests that IAA plays a novel role signaling between different E. huxleyi cell types, rather than between a bacteria and its algal host.

Keywords: Emiliania huxleyi; algae; auxin; cell type; coccolith; haptophyte; indole-3-acetic acid (IAA); phytohormone

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