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Sakata M, Seno M, Matsusaka H, et al. Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'. Breed Sci. 2016;66(3):425-33doi: 10.1270/jsbbs.15135.
Sakata, M., Seno, M., Matsusaka, H., Takahashi, K., Nakamura, Y., Yamagata, Y., Angeles, E. R., Mochizuki, T., Kumamaru, T., Sato, M., Enomoto, A., Tashiro, K., Kuhara, S., Satoh, H., & Yoshimura, A. (2016). Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'. Breeding science, 66(3), 425-33. https://doi.org/10.1270/jsbbs.15135
Sakata, Mitsukazu, et al. "Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'." Breeding science vol. 66,3 (2016): 425-33. doi: https://doi.org/10.1270/jsbbs.15135
Sakata M, Seno M, Matsusaka H, Takahashi K, Nakamura Y, Yamagata Y, Angeles ER, Mochizuki T, Kumamaru T, Sato M, Enomoto A, Tashiro K, Kuhara S, Satoh H, Yoshimura A. Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'. Breed Sci. 2016 Jun;66(3):425-33. doi: 10.1270/jsbbs.15135. Epub 2016 May 20. PMID: 27436953; PMCID: PMC4902460.
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Breed Sci. 2016 Jun;66(3):425-33. doi: 10.1270/jsbbs.15135. Epub 2016 May 20.

Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar 'Mizuhochikara'.

Breeding science

Mitsukazu Sakata, Mari Seno, Hiroaki Matsusaka, Kiyomi Takahashi, Yuki Nakamura, Yoshiyuki Yamagata, Enrique R Angeles, Toshihiro Mochizuki, Toshihiro Kumamaru, Masao Sato, Akiko Enomoto, Kosuke Tashiro, Satoru Kuhara, Hikaru Satoh, Atsushi Yoshimura

Affiliations

  1. Faculty of Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.
  2. Institute of Tropical Agriculture, Kyushu University , 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan.

PMID: 27436953 PMCID: PMC4902460 DOI: 10.1270/jsbbs.15135

Abstract

Rice bran oil is a byproduct of the milling of rice (Oryza sativa L.). It offers various health benefits and has a beneficial fatty acid composition. To increase the amount of rice bran as a sink for triacylglycerol (TAG), we developed and characterized new breeding materials with giant embryos. To induce mutants, we treated fertilized egg cells of the high-yielding cultivar 'Mizuhochikara' with N-methyl-N-nitrosourea (MNU). By screening M2 seeds, we isolated four giant embryo mutant lines. Genetic analysis revealed that the causative loci in lines MGE12 and MGE13 were allelic to giant embryo (ge) on chromosome 7, and had base changes in the causal gene Os07g0603700. On the other hand, the causative loci in lines MGE8 and MGE14 were not allelic to ge, and both were newly mapped on chromosome 3. The TAG contents of all four mutant lines increased relative to their wild type, 'Mizuhochikara'. MGE13 was agronomically similar to 'Mizuhochikara' and would be useful for breeding for improved oil content.

Keywords: N-methyl-N-nitrosourea (MNU); giant embryo; mutant; rice bran oil; triacylglycerol (TAG)

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