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Pravadali-Cekic S, Kocic D, Hua S, et al. Curtain Flow Column: Optimization of Efficiency and Sensitivity. J Vis Exp. 2016;(112)doi: 10.3791/53471.
Pravadali-Cekic, S., Kocic, D., Hua, S., Jones, A., Dennis, G., & Shalliker, A. (2016). Curtain Flow Column: Optimization of Efficiency and Sensitivity. Journal of visualized experiments : JoVE, (112), . https://doi.org/10.3791/53471
Pravadali-Cekic, Sercan, et al. "Curtain Flow Column: Optimization of Efficiency and Sensitivity." Journal of visualized experiments : JoVE vol. ,112 (2016). doi: https://doi.org/10.3791/53471
Pravadali-Cekic S, Kocic D, Hua S, Jones A, Dennis G, Shalliker A. Curtain Flow Column: Optimization of Efficiency and Sensitivity. J Vis Exp. 2016 Jun 12;(112). doi: 10.3791/53471. PMID: 27341443; PMCID: PMC4927787.
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J Vis Exp. 2016 Jun 12;(112). doi: 10.3791/53471.

Curtain Flow Column: Optimization of Efficiency and Sensitivity.

Journal of visualized experiments : JoVE

Sercan Pravadali-Cekic, Danijela Kocic, Stanley Hua, Andrew Jones, Gary Dennis, Andrew Shalliker

Affiliations

  1. School of Science and Health, University of Western Sydney.
  2. School of Science and Health, University of Western Sydney; [email protected].

PMID: 27341443 PMCID: PMC4927787 DOI: 10.3791/53471

Abstract

Active Flow Technology (AFT) is a form of column technology that increases the separation performance of a HPLC column through the use of a specially purpose built multiport end-fitting(s). Curtain Flow (CF) columns belong to the AFT suite of columns, specifically the CF column is designed so that the sample is injected into the radial central region of the bed and a curtain flow of mobile phase surrounding the injection of solute prevents the radial dispersion of the sample to the wall. The column functions as an 'infinite diameter' column. The purpose of the design is to overcome the radial heterogeneity of the column bed, and at the same time maximize the sample load into the radial central region of the column bed, which serves to increase detection sensitivity. The protocol described herein outlines the system and CF column set up and the tuning process for an optimized infinite diameter 'virtual' column.

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