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Onogi S, Shigemitsu H, Yoshii T, et al. In situ real-time imaging of self-sorted supramolecular nanofibres. Nat Chem. 2016;8(8):743-52doi: 10.1038/nchem.2526.
Onogi, S., Shigemitsu, H., Yoshii, T., Tanida, T., Ikeda, M., Kubota, R., & Hamachi, I. (2016). In situ real-time imaging of self-sorted supramolecular nanofibres. Nature chemistry, 8(8), 743-52. https://doi.org/10.1038/nchem.2526
Onogi, Shoji, et al. "In situ real-time imaging of self-sorted supramolecular nanofibres." Nature chemistry vol. 8,8 (2016): 743-52. doi: https://doi.org/10.1038/nchem.2526
Onogi S, Shigemitsu H, Yoshii T, Tanida T, Ikeda M, Kubota R, Hamachi I. In situ real-time imaging of self-sorted supramolecular nanofibres. Nat Chem. 2016 Aug;8(8):743-52. doi: 10.1038/nchem.2526. Epub 2016 May 30. PMID: 27442279.
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Nat Chem. 2016 Aug;8(8):743-52. doi: 10.1038/nchem.2526. Epub 2016 May 30.

In situ real-time imaging of self-sorted supramolecular nanofibres.

Nature chemistry

Shoji Onogi, Hajime Shigemitsu, Tatsuyuki Yoshii, Tatsuya Tanida, Masato Ikeda, Ryou Kubota, Itaru Hamachi

Affiliations

  1. Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
  2. Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan.
  3. Department of Biomolecular Science, Graduate School of Engineering, Gifu University, Gifu 501-1193, Japan.
  4. United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu 501-1193, Japan.
  5. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.

PMID: 27442279 DOI: 10.1038/nchem.2526

Abstract

Self-sorted supramolecular nanofibres-a multicomponent system that consists of several types of fibre, each composed of distinct building units-play a crucial role in complex, well-organized systems with sophisticated functions, such as living cells. Designing and controlling self-sorting events in synthetic materials and understanding their structures and dynamics in detail are important elements in developing functional artificial systems. Here, we describe the in situ real-time imaging of self-sorted supramolecular nanofibre hydrogels consisting of a peptide gelator and an amphiphilic phosphate. The use of appropriate fluorescent probes enabled the visualization of self-sorted fibres entangled in two and three dimensions through confocal laser scanning microscopy and super-resolution imaging, with 80 nm resolution. In situ time-lapse imaging showed that the two types of fibre have different formation rates and that their respective physicochemical properties remain intact in the gel. Moreover, we directly visualized stochastic non-synchronous fibre formation and observed a cooperative mechanism.

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