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Akasaka K, Ohrui H. Enatiomeric Separation of Branched Fatty Acids after Conversion with trans-2-(2,3-Anthracenedicarboximido)cyclohexanol, a Highly Sensitive Chiral Fluorescent Conversion Reagent. Biosci Biotechnol Biochem. 1999;63(7):1209-15doi: 10.1271/bbb.63.1209.
Akasaka, K., & Ohrui, H. (1999). Enatiomeric Separation of Branched Fatty Acids after Conversion with trans-2-(2,3-Anthracenedicarboximido)cyclohexanol, a Highly Sensitive Chiral Fluorescent Conversion Reagent. Bioscience, biotechnology, and biochemistry, 63(7), 1209-15. https://doi.org/10.1271/bbb.63.1209
Akasaka, K, and Ohrui, H. "Enatiomeric Separation of Branched Fatty Acids after Conversion with trans-2-(2,3-Anthracenedicarboximido)cyclohexanol, a Highly Sensitive Chiral Fluorescent Conversion Reagent." Bioscience, biotechnology, and biochemistry vol. 63,7 (1999): 1209-15. doi: https://doi.org/10.1271/bbb.63.1209
Akasaka K, Ohrui H. Enatiomeric Separation of Branched Fatty Acids after Conversion with trans-2-(2,3-Anthracenedicarboximido)cyclohexanol, a Highly Sensitive Chiral Fluorescent Conversion Reagent. Biosci Biotechnol Biochem. 1999;63(7):1209-15. doi: 10.1271/bbb.63.1209. PMID: 27380231.
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Biosci Biotechnol Biochem. 1999;63(7):1209-15. doi: 10.1271/bbb.63.1209.

Enatiomeric Separation of Branched Fatty Acids after Conversion with trans-2-(2,3-Anthracenedicarboximido)cyclohexanol, a Highly Sensitive Chiral Fluorescent Conversion Reagent.

Bioscience, biotechnology, and biochemistry

K Akasaka, H Ohrui

Affiliations

  1. a Division of Applied Life Science, Graduate School of Agricultural Science, Tohoku University.

PMID: 27380231 DOI: 10.1271/bbb.63.1209

Abstract

(1R,2R)-2-(2,3-Anthracenedicarboximido)cyclohexanol was synthesized as a highly sensitive chiral fluorescent conversion reagent. The diastereomeric derivatives of chiral branched fatty acids that had methyl ethyl chirality from the 2 to 12 position were separated into 2 peaks by reversed-phase HPLC and detected at the 10(-15) mole level by fluorometry.

Keywords: chiral branched fatty acid; chiral fluorescence conversion reagent; enantiomeric separation; high-performance liquid chromatography

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