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Zeppa L, Ambrosone L, Guerra G, et al. In Vivo Near-Infrared Fluorescence Imaging of Aqueous Humor Outflow Structures. J Ophthalmol. 2016;2016:8706564doi: 10.1155/2016/8706564.
Zeppa, L., Ambrosone, L., Guerra, G., Fortunato, M., & Costagliola, C. (2016). In Vivo Near-Infrared Fluorescence Imaging of Aqueous Humor Outflow Structures. Journal of ophthalmology, 20168706564. https://doi.org/10.1155/2016/8706564
Zeppa, L, et al. "In Vivo Near-Infrared Fluorescence Imaging of Aqueous Humor Outflow Structures." Journal of ophthalmology vol. 2016 (2016): 8706564. doi: https://doi.org/10.1155/2016/8706564
Zeppa L, Ambrosone L, Guerra G, Fortunato M, Costagliola C. In Vivo Near-Infrared Fluorescence Imaging of Aqueous Humor Outflow Structures. J Ophthalmol. 2016;2016:8706564. doi: 10.1155/2016/8706564. Epub 2016 May 24. PMID: 27313871; PMCID: PMC4895040.
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J Ophthalmol. 2016;2016:8706564. doi: 10.1155/2016/8706564. Epub 2016 May 24.

In Vivo Near-Infrared Fluorescence Imaging of Aqueous Humor Outflow Structures.

Journal of ophthalmology

L Zeppa, L Ambrosone, G Guerra, M Fortunato, C Costagliola

Affiliations

  1. Department of Ophthalmology, G. Moscati Hospital, 83100 Avellino, Italy.
  2. Department of Bioscience and Territory, University of Molise, Pesche, 86090 Isernia, Italy.
  3. Department of Medicine and Health Sciences, University of Molise, 86100 Campobasso, Italy.
  4. Department of Ophthalmology, Bambino Gesù Hospital, 00146 Rome, Italy.
  5. Department of Medicine and Health Sciences, University of Molise, 86100 Campobasso, Italy; IRCCS Neuromed, Pozzilli, 86077 Isernia, Italy.

PMID: 27313871 PMCID: PMC4895040 DOI: 10.1155/2016/8706564

Abstract

The aim of this study has been to visualize the aqueous outflow system in patients affected by primary open angle glaucoma. A solution of indocyanine green (ICG) plus high viscosity viscoelastic solution was injected into the Schlemm canal during surgery in 10 glaucomatous patients undergoing canaloplasty. Soon after injection of the dye the borders of the scleral flap were completely stained due to partial reflux caused by the intrachannel resistance; progression of the dye along the Schlemm canal starting from the site of injection was then visualized. The filling of the collector channels was observed only in the patent portions of the Schlemm canal. The only noticeable aqueous veins were located in correspondence of the quadrant in which both the Schlemm canal and the collectors were patent. Lastly, a retrograde filling, of glomerular-shaped structures, deepest to the Schlemm canal was observed in the quadrants where the pathway was functioning. Our findings show that injection of a mixture composed of ICG and viscoelastic solution into the Schlemm canal allows a clear visualization of the functioning portions of the conventional outflow pathway. In addition, a retrograde filling of structures presumably located into the iris was also recorded. Clinical Trial Registration. Our study is registered in ISRCTN registry, number 54005880, DOI 10.1186/ISRCTN54005880.

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