Display options
Cite
Clifton LA, Holt SA, Hughes AV, et al. An Accurate In Vitro Model of the . Angew Chem Weinheim Bergstr Ger. 2015;127(41):12120-12123doi: 10.1002/ange.201504287.
Clifton, L. A., Holt, S. A., Hughes, A. V., Daulton, E. L., Arunmanee, W., Heinrich, F., Khalid, S., Jefferies, D., Charlton, T. R., Webster, J. R., Kinane, C. J., & Lakey, J. H. (2015). An Accurate In Vitro Model of the . Angewandte Chemie (Weinheim an der Bergstrasse, Germany), 127(41), 12120-12123. https://doi.org/10.1002/ange.201504287
Clifton, Luke A, et al. "An Accurate In Vitro Model of the ." Angewandte Chemie (Weinheim an der Bergstrasse, Germany) vol. 127,41 (2015): 12120-12123. doi: https://doi.org/10.1002/ange.201504287
Clifton LA, Holt SA, Hughes AV, Daulton EL, Arunmanee W, Heinrich F, Khalid S, Jefferies D, Charlton TR, Webster JR, Kinane CJ, Lakey JH. An Accurate In Vitro Model of the . Angew Chem Weinheim Bergstr Ger. 2015 Oct 05;127(41):12120-12123. doi: 10.1002/ange.201504287. Epub 2015 Sep 01. PMID: 27346898; PMCID: PMC4871320.
Copy Download .nbib Format: NLM
Share it on

Angew Chem Weinheim Bergstr Ger. 2015 Oct 05;127(41):12120-12123. doi: 10.1002/ange.201504287. Epub 2015 Sep 01.

An Accurate In Vitro Model of the .

Angewandte Chemie (Weinheim an der Bergstrasse, Germany)

Luke A Clifton, Stephen A Holt, Arwel V Hughes, Emma L Daulton, Wanatchaporn Arunmanee, Frank Heinrich, Syma Khalid, Damien Jefferies, Timothy R Charlton, John R P Webster, Christian J Kinane, Jeremy H Lakey

Affiliations

  1. ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Oxford Campus, Didcot, Oxfordshire, OX11 OQX (UK).
  2. Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232 (Australia).
  3. Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH (UK).
  4. Department of Physics, Carnegie Mellon University, 5000 Forbes Ave. Pittsburgh, PA 15213 (USA); National Institute of Standards and Technology Center for Neutron Research, Gaithersburg, MD 20899 (USA).
  5. School of Chemistry, University of Southampton, Southampton SO17 1BJ (UK).

PMID: 27346898 PMCID: PMC4871320 DOI: 10.1002/ange.201504287

Abstract

Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.

Keywords: Antibiotika; Gram‐negative Bakterien; Membranen; Struktur‐Aktivitäts‐Beziehungen; Wirkstoff‐Forschung

References

  1. Int J Antimicrob Agents. 2012 Apr;39(4):283-94 - PubMed
  2. J Biol Chem. 2012 Jan 2;287(1):337-46 - PubMed
  3. Microbiol Rev. 1992 Sep;56(3):395-411 - PubMed
  4. J R Soc Interface. 2014 Sep 6;11(98):20140245 - PubMed
  5. J Mol Biol. 1988 Jun 5;201(3):497-506 - PubMed
  6. Infect Immun. 1988 Nov;56(11):2774-81 - PubMed
  7. J Phys Chem B. 2007 Mar 15;111(10):2477-83 - PubMed
  8. J Chem Theory Comput. 2012 Nov 13;8(11):4593-609 - PubMed
  9. Langmuir. 2015;31(1):404-12 - PubMed
  10. J R Soc Interface. 2013 Oct 16;10(89):20130810 - PubMed
  11. Langmuir. 2011 Jun 21;27(12):7698-707 - PubMed
  12. ACS Chem Biol. 2014 May 16;9(5):1172-7 - PubMed
  13. Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2390-5 - PubMed
  14. Nat Rev Microbiol. 2008 Dec;6(12):893-903 - PubMed
  15. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9147-51 - PubMed
  16. Soft Matter. 2009;5(13):2576-2586 - PubMed
  17. Innate Immun. 2014 May;20(4):350-63 - PubMed
  18. Mol Microbiol. 2014 May;92(3):440-52 - PubMed
  19. ACS Nano. 2012 Apr 24;6(4):3189-95 - PubMed
  20. J Mol Biol. 1997 Nov 28;274(2):222-36 - PubMed
  21. Langmuir. 2008 Mar 4;24(5):1989-99 - PubMed
  22. J Clin Invest. 1991 Oct;88(4):1080-91 - PubMed
  23. J Endotoxin Res. 2005;11(3):133-44 - PubMed

Publication Types

Grant support